Department of Respiratory Medicine, Center for Pathogen Biology and Infectious Diseases, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China.
Published: November 2022
AI Article Synopsis
Article Abstract
Background: (), as a pandemic foodborne pathogen, severely threatens food security and public health care worldwide, which evolves multiple bacterial virulence factors (such as listeriolysin O, LLO) for manipulating the immune response of -host interactions.
Methods: Hemolysis assay was employed to screen a potential LLO inhibitor and the underlying mechanisms were investigated using molecular dynamics (MD) simulation and oligomerization assay. The effects of candidates on immune response were examined by qRT-PCR and immunoblotting analysis. Histological analysis, ELISA assay and biochemistry detection were conducted to assess efficacy of candidates.
Results: In the present study, natural terpenoid atractylodin was characterized as an alternative drug candidate for the treatment of by the regulation of LLO function and host Nrf2/NLRP3 signaling pathway. Notably, infection model by also highlighted that atractylodin treatment provided effective therapeutic benefits, as evidenced by decreased bacterial burden and diminished inflammation. Congruently, the survival rate of -infection mice increased significantly from 10.0% to 40.0% by atractylodin treatment.
Conclusion: Collectively, our study showed for the first time that atractylodin has tremendous potential to attenuate pathogenicity by blocking LLO pore formation and mediating the suppression of inflammation and oxidative stress, providing a promising therapeutic strategy and broadening the applications of atractylodin against infection.
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