Dual transcriptome of and interplay in biofilms.

J Oral Microbiol

Departments of Obstetrics and Gynecology and Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY, USA.

Published: November 2022

Objective: To assess the interactions between  and during cariogenic biofilm formation.

Methods: The and duo-species biofilms were formed in 1% sucrose to mimic the high caries risk challenges. The biofilm structure was assessed using two-photon laser confocal microscopy. The transcriptome of 48h-biofilms was assessed by RNA-Seq. The expression of  and  virulence genes was examined via real-time reverse transcription-polymerase chain reaction.

Results: The morphogenesis of  duo-species biofilms was significantly altered when comparing to or single-species biofilm. Duo-species biofilms exhibited unique expression profile with a large number of differentially expressed genes (DEGs), including a higher expression of (acid-adaptive), (fungal cell wall chitin remodeling), and (cytotoxic oxygen radical destroying) (p < 0.05). KEGG pathway analyses further revealed that the majority of the up-regulated DEGs are related to microbial metabolism. Furthermore, the expressions of and key virulence genes (, C, , , , , ) were associated with sugar availability-related and time-related dynamics.

Conclusion: Cross-kingdom interactions impact  biofilm formations and dynamic expressions of virulence genes.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662060PMC
http://dx.doi.org/10.1080/20002297.2022.2144047DOI Listing

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Dual transcriptome of and interplay in biofilms.

J Oral Microbiol

November 2022

Departments of Obstetrics and Gynecology and Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY, USA.

Objective: To assess the interactions between  and during cariogenic biofilm formation.

Methods: The and duo-species biofilms were formed in 1% sucrose to mimic the high caries risk challenges. The biofilm structure was assessed using two-photon laser confocal microscopy.

View Article and Find Full Text PDF

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