Objective: To assess the interactions between and during cariogenic biofilm formation.
Methods: The and duo-species biofilms were formed in 1% sucrose to mimic the high caries risk challenges. The biofilm structure was assessed using two-photon laser confocal microscopy. The transcriptome of 48h-biofilms was assessed by RNA-Seq. The expression of and virulence genes was examined via real-time reverse transcription-polymerase chain reaction.
Results: The morphogenesis of duo-species biofilms was significantly altered when comparing to or single-species biofilm. Duo-species biofilms exhibited unique expression profile with a large number of differentially expressed genes (DEGs), including a higher expression of (acid-adaptive), (fungal cell wall chitin remodeling), and (cytotoxic oxygen radical destroying) (p < 0.05). KEGG pathway analyses further revealed that the majority of the up-regulated DEGs are related to microbial metabolism. Furthermore, the expressions of and key virulence genes (, C, , , , , ) were associated with sugar availability-related and time-related dynamics.
Conclusion: Cross-kingdom interactions impact biofilm formations and dynamic expressions of virulence genes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662060 | PMC |
| http://dx.doi.org/10.1080/20002297.2022.2144047 | DOI Listing |
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