Objective: To assess the interactions between and during cariogenic biofilm formation.
Methods: The and duo-species biofilms were formed in 1% sucrose to mimic the high caries risk challenges. The biofilm structure was assessed using two-photon laser confocal microscopy. The transcriptome of 48h-biofilms was assessed by RNA-Seq. The expression of and virulence genes was examined via real-time reverse transcription-polymerase chain reaction.
Results: The morphogenesis of duo-species biofilms was significantly altered when comparing to or single-species biofilm. Duo-species biofilms exhibited unique expression profile with a large number of differentially expressed genes (DEGs), including a higher expression of (acid-adaptive), (fungal cell wall chitin remodeling), and (cytotoxic oxygen radical destroying) (p < 0.05). KEGG pathway analyses further revealed that the majority of the up-regulated DEGs are related to microbial metabolism. Furthermore, the expressions of and key virulence genes (, C, , , , , ) were associated with sugar availability-related and time-related dynamics.
Conclusion: Cross-kingdom interactions impact biofilm formations and dynamic expressions of virulence genes.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662060 | PMC |
http://dx.doi.org/10.1080/20002297.2022.2144047 | DOI Listing |
Appl Environ Microbiol
October 2023
Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics (CMPG), Leuven, Belgium.
While the evolution of antimicrobial resistance is well studied in free-living bacteria, information on resistance development in dense and diverse biofilm communities is largely lacking. Therefore, we explored how the social interactions in a duo-species biofilm composed of the brewery isolates and influence the adaptation to the broad-spectrum antimicrobial sulfathiazole. Previously, we showed that the competition between these brewery isolates enhances the antimicrobial tolerance of .
View Article and Find Full Text PDFPathogens
August 2023
Department of Restorative Dentistry, School of Dentistry, Araraquara, São Paulo State University-UNESP, Araraquara 14801-903, SP, Brazil.
Nutrients
April 2023
Eastman Institute for Oral Health, University of Rochester Medical Center, Rochester, NY 14642, USA.
Ecological approaches can help to correct oral microbial dysbiosis and drive the advent and persistence of a symbiotic oral microbiome, which benefits long-term dental caries control. The aim of this study was to investigate the impact of the prebiotic Galacto-oligosaccharide (GOS) on the growth of probiotics 14,917 and its effect on the inhibitory ability of 14,917 against the growth of and in an in vitro model. Single-species growth screenings were conducted in TSBYE broth with 1% glucose and 1-5% GOS.
View Article and Find Full Text PDFArch Oral Biol
January 2023
Eastman Institute for Oral Health, University of Rochester Medical Center, Rochester, USA. Electronic address:
Objective: To assess the effect of Nystatin on Candida albicans and Streptococcus mutans duo-species biofilms using an in vitro cariogenic biofilm model.
Design: Biofilms were formed on saliva-coated hydroxyapatite discs under high sugar challenge (1 % sucrose and 1 % glucose), with inoculation of 10CFU/ml S. mutans and 10CFU/ml C.
J Oral Microbiol
November 2022
Departments of Obstetrics and Gynecology and Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY, USA.
Objective: To assess the interactions between and during cariogenic biofilm formation.
Methods: The and duo-species biofilms were formed in 1% sucrose to mimic the high caries risk challenges. The biofilm structure was assessed using two-photon laser confocal microscopy.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!