AI Article Synopsis
- The -group includes both common, typically non-harmful species often seen as contaminants and some highly virulent strains.
- This study examined 16 isolates from 15 patients identified using MALDI-TOF MS, finding varied species classifications through whole genome sequencing, with some lacking known virulence plasmids.
- The research underscores the challenges in accurately identifying avirulent species in the -group with standard diagnostic methods, suggesting that advanced techniques like WGS and specialized databases could improve species identification.
Article Abstract
The -group () includes common, usually avirulent species, often considered contaminants of patient samples in routine microbiological diagnostics, as well as the highly virulent . Here we describe 16 isolates from 15 patients, identified as -group using a MALDI-TOF MS standard database. Whole genome sequencing (WGS) analysis identified five of the isolates as species not carrying the typical virulence plasmids pXO1 and pXO2, four isolates as three as , two as , one as , and one isolate represents a previously undefined species of ( sp. nov.). More detailed analysis using alternative MALDI-TOF MS databases, biochemical phenotyping, and diagnostic PCRs, gave further conflicting species results. These cases highlight the difficulties in identifying avirulent within the -group using standard methods. WGS and alternative MALDI-TOF MS databases offer more accurate species identification, but so far are not routinely applied. We discuss the diagnostic resolution and discrepancies of various identification methods.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641004 | PMC |
| http://dx.doi.org/10.1016/j.nmni.2022.101040 | DOI Listing |
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