AI Article Synopsis
- Active stresses in F-actin structures help direct crucial cell functions like division and migration, but how these stresses are generated based on different architectures needs more investigation.
- In this study, various F-actin networks were created in a lab, using different methods to alter their architecture (from branched to bundled) and observe effects on stress generation.
- Results showed that bundled networks promoted stress build-up and contraction, while branched networks limited stress accumulation because the thick filaments moved less freely due to the high density and specific shapes of the F-actin.
Article Abstract
Active stresses are generated and transmitted throughout diverse F-actin architectures within the cell cytoskeleton, and drive essential behaviors of the cell, from cell division to migration. However, while the impact of F-actin architecture on the transmission of stress is well studied, the role of architecture on the ab initio generation of stresses remains less understood. Here, we assemble F-actin networks in vitro, whose architectures are varied from branched to bundled through F-actin nucleation via Arp2/3 and the formin mDia1. Within these architectures, we track the motions of embedded myosin thick filaments and connect them to the extent of F-actin network deformation. While mDia1-nucleated networks facilitate the accumulation of stress and drive contractility through enhanced actomyosin sliding, branched networks prevent stress accumulation through the inhibited processivity of thick filaments. The reduction in processivity is due to a decrease in translational and rotational motions constrained by the local density and geometry of F-actin.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669029 | PMC |
| http://dx.doi.org/10.1038/s41467-022-34715-6 | DOI Listing |
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