Correlation of miR-24-3p and miR-595 expression with and genes in PBMCs of patients with coronary artery disease.

Inflammation has been well recognized to play an important role in developing coronary artery disease (CAD). By regulating essential genes in this pathway post-transcriptionally, MicroRNAs (miRNAs) may help or hinder the development of atherosclerotic lesions. The aim of this study was to investigate the expression of miR-24-3p, miR-595, , , , , and in the peripheral blood mononuclear cells (PBMCs) of CAD and control groups and to examine whether any correlation exists between the expression of miRs and genes in CAD group. A total of 168 subjects (84 CAD subjects and 84 control subjects) were examined in this research. Expression levels of miR-24-3p, miR-595, CCL3, CCL4, IL-1β, and in PBMCs were measured using the real-time PCR technique. A comparison of the CAD group with the control group indicated significantly increased expression levels of , and (Fold Change (FC)=4, =0.009; FC=2.9, =0.01; FC=1.8, =0.019, respectively) and remarkably reduced expression levels of and (FC=-1.4, =0.03 and FC=-5.9, =0.001, respectively). Moreover, the expression levels of miR-24-3p downregulated (FC=-2.5, =0.005) and miR-595 upregulated (FC=1.9, =0.009) in the CAD group. There was a statistical correlation between the number of clogged arteries with expression levels of miR-24-3p, miR-595, , , , , and in the CAD group. Also, there was a statistical correlation between expression levels of miR-24-3p and miR-595 with , , , , and gene expression in the CAD group. In CAD patients, decreased expression of miR-24-3p and increased expression of miR-595 may aid the progression of atherosclerotic plaques by regulating , , , , and gene expression.