The oncolytic activity of nontoxic spores in breast cancer.

Hypoxia context is highly specific for tumors and represents a unique niche which is not found elsewhere in the body. is an obligate anaerobic bacterium. It has a potential to treat tumors. The aim of this study was to produce the nontoxic spores and to investigate its oncolytic effect on breast cancer in mice model. Primarily, the lethal toxin gene in i type B was removed. Colonies were isolated using PCR testing. To assure the removal of alpha-toxin, plasmid extraction and in vivo assay were conducted. Next, to treat breast cancer model in different sizes of tumors, a single dose of spores of nontoxic was tested. The results denoted that nontoxic lost lethal toxin and a--ppeared to be safe. For smaller than 1000 mm tumors, a single dose of nontoxic was able to cure 100% of mice bearing breast tumors. Hence the mice remained free of tumor relapse. Tumors larger than 1000 mm were not cured by a single dose- of nontoxic treatment. The experiment concluded that the nontoxic might be a suitable and safe candidate, a novel therapeutic approach to encounter such hypoxic regions in the center of tumors. Research also showed that bacteriolytic therapy by nontoxic could lead to regression in small tumor.