In this study alginate-based microbubbles with a raspberry-like or core-shell-like morphology and with an average particle size of 553.6 ± 69.6 μm were synthesized; this was done through a novel procedure of transforming the structure with a 40 kHz ultrasonication which also stimulated the release of the components inside. Through the use of the electrospray technique in conjunction with agitation processes, components such as shikonin (SHK) and indocyanine green (ICG) were simultaneously encapsulated in alginate microbubbles to produce SHK-ICG alginate microbubbles; these microbubbles had half-maximal inhibitory concentrations of approximately 2.08 and 4.43 μM toward CP70 and SKOV3 ovarian cancer-cell lines, respectively, in an cell model. Moreover, these SHK-ICG alginate microbubbles enhanced brightness by 2.5 fold in ultrasound imaging relative to CaCl medium only. In conclusion, SHK-ICG alginate microbubbles have promise for use in theranostics.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641574 | PMC |
| http://dx.doi.org/10.1039/d2ra06298d | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Remote-Controlled Gene Delivery in Coaxial 3D-Bioprinted Constructs using Ultrasound-Responsive Bioinks.
Cell Mol Bioeng
October 2024
Biomedical Engineering Department, Oregon Health and Science University, Portland, OR 97201 USA.
Introduction: Coaxial 3D bioprinting has advanced the formation of tissue constructs that recapitulate key architectures and biophysical parameters for in-vitro disease modeling and tissue-engineered therapies. Controlling gene expression within these structures is critical for modulating cell signaling and probing cell behavior. However, current transfection strategies are limited in spatiotemporal control because dense 3D scaffolds hinder diffusion of traditional vectors.
View Article and Find Full Text PDFT Cell Activators Exhibit Distinct Downstream Effects on Chimeric Antigen Receptor T Cell Phenotype and Function.
Immunohorizons
June 2024
Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD.
T cell activation is an essential step in chimeric Ag receptor (CAR) T (CAR T) cell manufacturing and is accomplished by the addition of activator reagents that trigger the TCR and provide costimulation. We explore several T cell activation reagents and examine their effects on key attributes of CAR T cell cultures, such as activation/exhaustion markers, cell expansion, gene expression, and transduction efficiency. Four distinct activators were examined, all using anti-CD3 and anti-CD28, but incorporating different mechanisms of delivery: Dynabeads (magnetic microspheres), TransAct (polymeric nanomatrix), Cloudz (alginate hydrogel), and Microbubbles (lipid membrane containing perfluorocarbon gas).
View Article and Find Full Text PDFHarnessing the potential of in-situ, electrically generated microbubbles via nickel foam for enhanced, low energy membrane fouling control.
Water Res
February 2024
Department of Chemical and Environmental Engineering, Yale University, New Haven CT 06511, USA. Electronic address:
For membrane-based, water treatment technologies, fouling remains a significant challenge for pressure-driven processes. While many antifouling strategies have been proposed, there remains significant room for improved efficiency. Direct application of microbubbles (MBs) at a membrane surface offers a promising approach for managing interfacial fouling through continuous physical interaction(s).
View Article and Find Full Text PDFTreatment of high-salinity brine containing dissolved organic matters by vacuum membrane distillation: A fouling mitigation approach via microbubble aeration.
J Environ Manage
September 2023
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China.
Article Synopsis
- A laboratory-scale vacuum membrane distillation (VMD) system with microbubble aeration (MBA) was developed to treat high-salinity brine containing organic materials.
- Initial tests showed that the presence of model organics did not significantly affect the permeate flux despite causing some fouling on the membrane surface.
- The use of MBA was effective in reducing membrane fouling and removing about 50% of salt precipitation, while consuming only 2.2% of total energy, showcasing its potential as a solution for enhancing membrane performance in VMD systems.
Transformation of theranostic alginate-based microbubbles from raspberry-like to core-shell-like microbubbles and studies.
RSC Adv
November 2022
Department of Obstetric and Gynecology, Tri-Service General Hospital, National Defense Medical Centre Taiwan.
In this study alginate-based microbubbles with a raspberry-like or core-shell-like morphology and with an average particle size of 553.6 ± 69.6 μm were synthesized; this was done through a novel procedure of transforming the structure with a 40 kHz ultrasonication which also stimulated the release of the components inside.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!