Methoprene, a juvenile hormone (JH) analog, is widely used for insect control, but its mode of action is not known. To study methoprene action in the yellow fever mosquito, , the (ecdysone-induced transcription factor) was knocked out using the CRISPR-Cas9 system. The E93 mutant pupae retained larval tissues similar to methoprene-treated insects. These insects completed pupal ecdysis and died as pupa. In addition, the expression of transcription factors, and (), increased and that of programmed cell death (PCD) and autophagy genes decreased in mutants. These data suggest that methoprene functions through JH receptor, methoprene-tolerant, and induces the expression of , which suppresses the expression of , resulting in a block in PCD and autophagy of larval tissues. Failure in the elimination of larval tissues and the formation of adult structures results in their death. These results answered long-standing questions on the mode of action of methoprene.
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| http://dx.doi.org/10.1089/crispr.2022.0066 | DOI Listing |
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