Effects of PFOS at ng/L levels on photostability of extracellular polymeric substances under solar irradiation by fluorescence and infrared spectroscopy.

The ubiquitous EPS (extracellular polymeric substances), as a type of dissolved organic carbon, plays a key role in carbon cycling in water environment. When EPS meet the omnipresent PFOS (perfluorooctane sulfonate), they must interact with each other and exert profound effect on behavior and fate of both, which is still not well known. We hypothesized that the highly persistent PFOS at real environmental levels may significantly influence behavior of EPS under solar irradiation which may retard carbon turnover. In this study, 3D-EEM fluorescence spectroscopy and FTIR spectroscopy were used to probe responses of composition and structure of EPS under solar irradiation in the absence and presence of PFOS (5-500 ng/L). The experimental results showed that PFOS at ng/L levels significantly affected responses of EPS to sunlight irradiation and the effects were dependent on the components in EPS. Photostability of humic-like substances was significantly increased in the presence of PFOS; Degradation and unfolding of proteins induced by solar light were reduced by PFOS. In addition, degradation of both hydrophilic and hydrophobic functional groups by sunlight was inhibited by PFOS. The novel findings provide new insights for assessing the environmental behavior of EPS and PFOS and understanding the effect of PFOS on carbon cycling in water environments.