AI Article Synopsis
- A high-performance liquid chromatography (HPLC) method has been created for analyzing brevetoxins, neurotoxins from harmful algae, using a specialized cholesterol column.
- Various experimental conditions were tested to optimize the protocol, including differences in elution methods, organic solvents, and temperatures.
- A separate analysis method for brevenal and brevenol was established, as brevenal shows promise as a treatment for chronic respiratory diseases, aiming to provide a flexible protocol for labs to validate and utilize.
Article Abstract
A high-performance liquid chromatography protocol for the analysis of brevetoxins has been developed using a silica hydride-based cholesterol column. Brevetoxins are neurotoxins produced by harmful algae that have additional potential as drugs for a number of illnesses/diseases. To develop the optimum conditions, a number of different experimental approaches were tested. These include isocratic and gradient elution, different organic mobile phase components, and temperature variations. A separate protocol was developed for the compounds brevenal and brevenol, also produced by the same algae that make brevetoxins. Brevenal is a natural product under investigation as a therapy for chronic respiratory diseases, such as cystic fibrosis or asthma. The goal of this study was to provide a protocol for the analysis of these compounds that could be further developed into a validated method depending on a particular laboratory's capabilities and to highlight some of the unique features of the cholesterol stationary phase.
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| http://dx.doi.org/10.1002/jssc.202200666 | DOI Listing |
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