AI Article Synopsis
- Tyrosinase inhibitors can help protect the liver from damage caused by tyrosinase, making it important to find these natural inhibitors effectively.
- In this study, a plant called Isodon excisoides was extracted using ultrasound, and a new screening method combined with advanced chromatography techniques was used to identify potential tyrosinase inhibitors.
- Seven components were found to have high binding affinity to tyrosinase, including lasiokaurin and excisanin B, which showed better inhibition than the known inhibitor resveratrol, suggesting a promising new approach to discovering bioactive compounds from plants.
Article Abstract
Tyrosinase inhibitors can alleviate the harm to the liver caused by tyrosinase. How to effectively screen out natural tyrosinase inhibitors becomes a focus. In this study, Isodon excisoides was first extracted with the ultrasound optimized by response surface methodology. Then, a method combined ultrafiltration with ultra-liquid chromatography mass spectrometry (UHPLC/MS) was built to screen and identify tyrosinase inhibitors. The binding energies of active ingredients to tyrosinase were calculated by molecular docking. The reliability of the results was validated by the IC of enzyme inhibition assay. As a result, the binding energies of 7 components including excisanin B, lasiokaurin, rabdophyllin G, rabdoserrin B, rabdosin D, rabdosinate and weisiensin were lower than that of resveratrol. It was indicated that these components had high tyrosinase inhibitory activity. The IC values of lasiokaurin and excisanin B were 177 and 142 μmol/mL, which were less than that of resveratrol (183 μmol/mL). It showed that this way was simple, rapid, reliable and effective, which provided a new strategy to screen natural bioactive compounds from plants.
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| http://dx.doi.org/10.1002/cbdv.202200748 | DOI Listing |
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