Functionalized antibiofouling membranes have attracted increasing attention in water and wastewater treatment. Among them, contact-killing antibiofouling membranes deliver a long-lasting effect with no leaching or release, thus providing distinctive advantages. However, the antibiofouling mechanism especially in the vicinity of the membrane surface remains unclear. Herein, we demonstrate that mazEF-mediated programmed cell death (PCD) is critical for the antibiofouling behaviors of quaternary ammonium compounds modified membranes (QM). The viability of wild type Escherichia coli (WT E. coli) upon exposure to QM for 1 h was decreased dramatically (31.5 ± 1.4% of the control). In contrast, the bacterial activity of E. coli with the knockout of mazEF gene (KO E. coli) largely remained (85.8 ± 5.2%). Through addition of quorum sensing factor, i.e., extracellular death factor (EDF), the antibacterial activity was significantly enhanced in a dilute culture, indicating that the density-dependent bacterial communication played an important role in the mazEF-mediated PCD system in biofouling control. Long-term study further showed that QM exhibited a better antibiofouling performance to treat feedwater containing WT E. coli, especially when EDF was dosed. Results of this study suggested that the bacteria on the membrane surface subject to contact killing could modulate the population growth in the vicinity via quorum-sensing mazEF-mediated PCD, paving a way to develop efficient antibiofouling materials based on contact-killing scenarios.
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http://dx.doi.org/10.1016/j.watres.2022.119319 | DOI Listing |
Environ Pollut
May 2024
Groundwater Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, 518055, China; Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, 518055, China; Shenzhen Key Laboratory of Ecological Remediation and Carbon Sequestration, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, 518055, China; Tsinghua University-Kunming Joint Research Center for Dianchi Plateau Lake, Tsinghua University, Beijing, 100084, China. Electronic address:
β-cyclocitral (BCC) is an odorous compound that can be produced by bloom-forming cyanobacteria, for example, Microcystis aeruginosa. BCC has been proposed to explain the rapid decline of cyanobacterial blooms in natural water bodies due to its lytic effects on cyanobacteria cells. However, few insights have been gained regarding the mechanisms of its lethality on cyanobacteria.
View Article and Find Full Text PDFWater Res
December 2022
State Key Laboratory of Pollution Control and Resource Reuse, Shanghai Institute of Pollution Control and Ecological Security, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address:
Functionalized antibiofouling membranes have attracted increasing attention in water and wastewater treatment. Among them, contact-killing antibiofouling membranes deliver a long-lasting effect with no leaching or release, thus providing distinctive advantages. However, the antibiofouling mechanism especially in the vicinity of the membrane surface remains unclear.
View Article and Find Full Text PDFJ Basic Microbiol
December 2016
School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, India.
Toxin-antitoxins systems (TAS) are prokaryotic operons containing two small overlapping genes which encode two components referred to as toxin and antitoxin. Involvement of TAS in bacterial programmed cell death (PCD) is highly controversial. MazEF, a typical type II TAS, is particularly implicated in mediating PCD in Escherichia coli.
View Article and Find Full Text PDFCrit Rev Microbiol
February 2015
School of Chemical and Biotechnology, SASTRA University, Thanjavur , India and.
Toxin-antitoxin (TA) systems consist of a bicistronic operon, encoding a toxin and an antitoxin. They are widely distributed in the prokaryotic kingdom, often in multiple numbers. TAs are implicated in contradicting phenomena of persistence and programmed cell death (PCD) in bacteria.
View Article and Find Full Text PDFPLoS Biol
July 2012
Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
In eukaryotes, the classical form of programmed cell death (PCD) is apoptosis, which has as its specific characteristics DNA fragmentation and membrane depolarization. In Escherichia coli a different PCD system has been reported. It is mediated by the toxin-antitoxin system module mazEF.
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