sp. nov., isolated from caecal contents of domestic pigs ().

Seven -like isolates were cultured from caecal contents of 100 domestic pigs () sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in sp. in 2015. The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small -like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates. Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive. They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of . Three strains (11154-15, 14348-15 and 16470-15) underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to . Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described species (ANI ≤90.6 %). The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to . The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel species for which the name sp. nov. is suggested, with strain 11154-15 (= DSM 113688 = CCUG 76053) as the type strain.