AI Article Synopsis
- The study investigates the antibacterial effects of silver (Ag) and copper (Cu) nanoparticles (NPs) against methicillin-resistant bacteria, comparing them to traditional metal salt solutions.
- NPs were created using nanosecond laser ablation and were characterized using various techniques like electron microscopy and spectroscopy to analyze their properties and effectiveness.
- The results highlighted that Ag and Cu NPs, along with metal salts, enhance bacterial membrane fluidity and cause cell damage through ion release and other mechanisms, leading to bacterial death, as validated by spectroscopic and microscopy techniques.
Article Abstract
We report the bactericidal effect of Ag and Cu NPs with different concentrations on methicillin-resistant strain in comparison to the effect of AgNO and CuCl solutions, characterized by microbiological tests, TEM and Fourier-transform infrared spectroscopy. NPs were produced by nanosecond laser ablation in distilled water and characterized by scanning electron microscopy, UV-vis, energy dispersive X-ray, FT-IR spectroscopy, as well as X-ray diffraction, dynamic light scattering size and zeta-potential measurements. Microbiological tests showed antibacterial activity of NPs and metal ion-containing salts. Comparative FT-IR spectroscopy of bacteria, treated with metal NPs and salts, showed the broadening of amide I and II bands, a CH-related peak and its frequency decrease, indicating the increase of membrane fluidity. The main mechanisms of the antibacterial effect were proposed: Ag and Cu NPs release ions and ROS, which result in lipid peroxidation; AgNO forms precipitates on the cell surface, which lead to the mechanical rupture of the membrane and subsequent possible penetration of the precipitates in the emerged damaged spots, complete destruction of the membrane and bacterial death; Cu ions from the CuCl solution cause damage to phosphorus- and sulfur-containing biomolecules, which leads to disruption of intracellular biochemical processes. The theories were confirmed by FT-IR spectroscopy and TEM.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657403 | PMC |
| http://dx.doi.org/10.3390/nano12213857 | DOI Listing |
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