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A Hydroxytricyanopyrrole-Based Fluorescent Probe for Sensitive and Selective Detection of Hypochlorous Acid. | LitMetric

A Hydroxytricyanopyrrole-Based Fluorescent Probe for Sensitive and Selective Detection of Hypochlorous Acid.

Molecules

Key Laboratory of Basic Pharmacology of the Ministry of Education and Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, Zunyi Medical University, No. 6 West Xuefu Road, Xinpu District, Zunyi 563000, China.

Published: October 2022

AI Article Synopsis

  • Hypochlorous acid (HOCl) plays a crucial role in inflammation and disease, but its detection in biological systems is essential for understanding its effects.
  • Researchers developed a fluorescent probe called HTCP-NTC that specifically detects trace amounts of HOCl and emits strong fluorescence, with a significant sensitivity of 21.7 nM.
  • HTCP-NTC provides rapid and real-time monitoring of HOCl levels in living cells, making it a promising tool for exploring the link between inflammation and HOCl.

Article Abstract

Hypochlorous acid (HOCl) is a reactive substance that reacts with most biomolecules and is essential in physiological and pathological processes. Abnormally elevated HOCl levels may cause inflammation and other disease responses. To further understand its key role in inflammation, HOCl must be detected in situ. Here, we designed a hydroxytricyanopyrrole-based small-molecule fluorescent probe (HTCP-NTC) to monitor and identify trace amounts of HOCl in biological systems. In the presence of HOCl, HTCP-NTC released hydroxyl groups that emit strong fluorescence covering a wide wavelength range from the visible to near-infrared region owing to the resumption of the intramolecular charge transfer process. Additionally, HTCP-NTC demonstrated a 202-fold fluorescence enhancement accompanied by a large Stokes shift and a low detection limit (21.7 nM). Furthermore, HTCP-NTC provided a rapid response to HOCl within 18 s, allowing real-time monitoring of intracellular HOCl. HTCP-NTC exhibited rapid kinetics and biocompatibility, allowing effective monitoring of the exogenous and endogenous HOCl fluctuations in living cells. Finally, based on fluorescence imaging, HTCP-NTC is a potential method for understanding the relationship between inflammation and HOCl.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656901PMC
http://dx.doi.org/10.3390/molecules27217237DOI Listing

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