Steric exclusion chromatography (SXC) is a promising purification method for biological macromolecules such as the Orf virus (ORFV) vector. The method's principle is closely related to conventional polyethylene glycol (PEG) precipitation, repeatedly implementing membranes as porous chromatographic media. In the past decade, several purification tasks with SXC showed exceptionally high yields and a high impurity removal. However, the effect of varying process parameters, on the precipitation success and its limitations to SXC, is not yet well understood. For this reason, the precipitation behavior and SXC adaptation for ORFV were investigated for the PEG/ORFV contact time, the membranes pore size, and the type and concentration of ions. All three parameters influenced the ORFV recoveries significantly. A small pore size and a long contact time induced filtration effects and inhibited a full virus recovery. The application of salts had complex concentration-dependent effects on precipitation and SXC yields, and ranged from a complete prevention of precipitation in the presence of kosmotropic substances to increased efficiencies with Mg ions. The latter finding might be useful to reduce PEG concentrations while maintaining high yields. With this knowledge, we hope to clarify several limitations of SXC operations and improve the tool-set for a successful process adaptation.
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http://dx.doi.org/10.3390/membranes12111070 | DOI Listing |
Water Res
December 2024
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:
Micropollutants (MPs) in aquaculture water are directly related to human health, but largely overlooked. The conventional water treatment technologies could not effectively remove MPs, and new technologies have been pursued with high MP removal rate, low cost and small footprint. This paper reported the first endeavor of using layer-by-layer (LBL) hollow fiber (HF) nanofiltration (NF) membranes to treat real aquaculture water.
View Article and Find Full Text PDFComput Struct Biotechnol J
December 2024
Department of Physics, Sapienza University, Piazzale Aldo Moro 5, 00185, Rome, Italy.
Enhanced stabilization of protein structures via the presence of inert osmolytes is a key mechanism adopted both by physiological systems and in biotechnological applications. While the intrinsic stability of proteins is ultimately fixed by their amino acid composition and organization, the interactions between osmolytes and proteins together with their concentrations introduce an additional layer of complexity and in turn, a method of modulating protein stability. Here, we combined experimental measurements with molecular dynamics simulations and graph-theory-based analyses to predict the stabilizing/destabilizing effects of different kinds of osmolytes on proteins during heat-mediated denaturation.
View Article and Find Full Text PDFSmall
December 2024
Radiochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, India.
Molecular layer deposition (MLD) of ethylene glycol-alucone (EG-alucone) on the Nafion cation exchange membrane is investigated to understand its impact on the morphology of the composite and consequent enhancement of ion transport selectivity. X-ray photoelectron spectroscopy, scanning electron microscopy, Density functional theory, and Doppler broadening positron annihilation spectroscopy are comprehensively employed to examine the morphology of the composite, particularly the engineered interface between EG-alucone and Nafion. These studies reveal the diffusion and subsequent reaction of the Lewis-acidic trimethyl aluminum precursor with the polymer substrate during MLD.
View Article and Find Full Text PDFJ Chromatogr A
November 2024
College of Pharmaceutical Science, Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan University of Chinese Medicine, Kunming 650500, China. Electronic address:
Restricted-access materials (RAMs) allow biological samples to directly enter the chromatographic column for analysis owing to the steric exclusion function ability for biomolecules and extraction function for small-molecule analytes, which promoting the development of rapid, efficient, and automated in vivo drug analysis. Few reports on chiral RAMs that have been used to analyze enantiomers and positional isomers in serum by direct injection in currently. In this study, a chiral porous organic cage material RCC3 was innovatively introduced into the inner surface of silica gel and modified the outer surface with polyethylene glycol to prepare a novel type of chiral RAM-RCC3, and reported the use of chiral RAM-RCC3 as a stationary phase for the separation of chiral compounds and positional isomers in blank serum using high-performance liquid chromatography.
View Article and Find Full Text PDFWater Res
January 2025
School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; National Observation and Research Station of Erhai Lake Ecosystem in Yunnan, Shanghai Jiao Tong University Yunnan Dali Research Institute, Dali, Yunnan 671006, China. Electronic address:
Rapid development of semiconductor manufacturing and photovoltaic industry leads to significant generation of fluoride-rich and silica-rich wastewaters. Due to the emphasis on circular economy and resource recovery, there is a shift from regarding wastewater as waste to a recoverable resource. In this study, we present a uniquely designed dual-ion permeation Janus membrane (DPM)-assisted element reconstitution system (MERS) for selective recovery of high-value fluorosilicates from fluoride-rich and silica-rich wastewaters.
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