Genome-Wide Identification of AP2/ERF Transcription Factor Family and Functional Analysis of Associated with Abiotic Stress in .

Int J Mol Sci

Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China.

Published: November 2022

AI Article Synopsis

  • The study identifies 120 members of the family in plants, categorized into four subfamilies based on phylogenetic analysis.
  • It reveals that each gene contains at least one AP2 domain and shows varied motif distributions, with significant cis-elements linked to hormone signaling and stress responses.
  • The research highlights the role of a specific gene in drought stress, showing it interacts with DREB2A and inhibits an enzyme's expression in response to ABA, providing insights into the gene's biological function in stress response.

Article Abstract

() family plays important roles in reproductive development, stress responses and hormone responses in plants. However, family has not been systematically studied in . In this study, 120 family members were identified for the first time in , which were divided into four groups (, , and subfamily) according to phylogenetic analysis. Gene structures and conserved motif analysis showed that each family gene contained at least one AP2 domain, and the distribution of motifs varied among subfamilies. Cis-element analysis indicated that genes contained abundant cis-elements related to hormone signaling and stress response. To further identify potential genes involved in drought stress, 12 genes were selected to detect their expression under drought treatment through qRT-PCR analysis and , a nuclear localized ethylene-responsive transcription factor, showed a strong response to PEG treatment. Overexpression of in showed sensitivity to ABA. Molecular, biochemical and genetic assays indicated that interacts with DREB2A and directly inhibits the expression of P5CS1 in response to the ABA signal. Taken together, our study provided a molecular basis for the intensive study of genes and revealed the biological function of involved in the ABA signal.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659080PMC
http://dx.doi.org/10.3390/ijms232113603DOI Listing

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