Six α-amylase/subtilisin inhibitor genes () were identified from mulberry (). In this study, bioinformatics and expression pattern analysis of six were performed to determine their roles in resistance to . The expression of all six was significantly increased under infection. , which responded strongly to , was overexpressed in and mulberry. The resistance of and mulberry overexpressing gene to was significantly improved, the catalase (CAT) activity was increased, and the malondialdehyde (MDA) content was decreased after inoculation with . At the same time, HO and O levels were reduced in transgenic , reducing the damage of ROS accumulation to plants. In addition, transgenic increased the expression of the salicylic acid (SA) pathway-related gene . This study provides an important reference for further revealing the function of α-amylase/subtilisin inhibitors.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656013PMC
http://dx.doi.org/10.3390/ijms232113372DOI Listing

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