Six α-amylase/subtilisin inhibitor genes () were identified from mulberry (). In this study, bioinformatics and expression pattern analysis of six were performed to determine their roles in resistance to . The expression of all six was significantly increased under infection. , which responded strongly to , was overexpressed in and mulberry. The resistance of and mulberry overexpressing gene to was significantly improved, the catalase (CAT) activity was increased, and the malondialdehyde (MDA) content was decreased after inoculation with . At the same time, HO and O levels were reduced in transgenic , reducing the damage of ROS accumulation to plants. In addition, transgenic increased the expression of the salicylic acid (SA) pathway-related gene . This study provides an important reference for further revealing the function of α-amylase/subtilisin inhibitors.
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http://dx.doi.org/10.3390/ijms232113372 | DOI Listing |
BMC Mol Cell Biol
January 2025
Department of Biological Sciences, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Background: The Hippo signaling pathway involves a kinase cascade that controls phosphorylation of the effector proteins YAP and TAZ, leading to regulation of cell growth, tissue homeostasis, and apoptosis. Morusin, a compound extracted from Morus alba, has shown potential in cancer therapy by targeting multiple signaling pathways, including the PI3K/Akt/mTOR, JAK/STAT, MAPK/ERK, and apoptosis pathways. This study explores the effects of morusin on YAP activation and its implications for apoptosis resistance.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Faculty of Fiber Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan. Electronic address:
Mulberry silk (Bombyx mori) and eri silk (Samia/Philosamia ricini) are widely used silks. Eri silk is a wild silk that contains an arginine-glycine-aspartic acid tripeptide sequence within its structure, making it a potential and sustainable biomaterial. However, its poor solubility using conventional methods has resulted in limited research compared with that of mulberry silk fibroin.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Bud dormancy is a critical adaptive trait in woody plants, essential for enduring harsh winter conditions. The relationship between bud break timing and cold resistance is complex and has been a subject of debate. This study utilizes a Genome-Wide Association Study (GWAS) on 201 natural mulberry populations to identify the gene, which shows the strongest association with bud break timing.
View Article and Find Full Text PDFFront Plant Sci
December 2024
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Xylem plasticity is important for trees to coordinate hydraulic efficiency and safety under changing soil water availability. However, the physiological and transcriptional regulations of cambium on xylem plasticity are not well understood. In this study, mulberry saplings of drought-resistant Wubu and drought-susceptible Zhongshen1 were subjected to moderate or severe drought stresses for 21 days and subsequently rewatered for 12 days.
View Article and Find Full Text PDFMicromachines (Basel)
October 2024
Shaanxi Key Laboratory of Nano-Materials and Technology, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Flexible pressure sensors based on paper have attracted considerable attention owing to their good performance, low cost, and environmental friendliness. However, effectively expanding the detection range of paper-based sensors with high sensitivities is still a challenge. Herein, we present a paper-based resistive pressure sensor with a sandwich structure consisting of two electrodes and three sensing layers.
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