The present study introduces a unique BL signature imaging system with novel CTZ analogues named "C-series." Nine kinds of C-series CTZ analogues were first synthesized, and BL intensity patterns and spectra were then examined according to the marine luciferases. The results show that the four CTZ analogues named C3, C4, C6, and C7, individually or collectively luminesce with completely distinctive BL spectral signatures and intensity patterns according to the luciferases: luciferase (RLuc), NanoLuc, and artificial luciferase (ALuc). The signatural reporters were multiplexed into a multi-reporter system comprising RLuc8.6-535SG and ALuc16. The usefulness of the signatural reporters was further determined with a multi-probe system that consists of two single-chain probes embedding RLuc8 and ALuc23. This study is a great addition to the study of conventional bioassays with a unique methodology, and for the specification of each signal in a single- or multi-reporter system using unique BL signatures and patterns of reporter luciferases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658928 | PMC |
| http://dx.doi.org/10.3390/ijms232113047 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regiospecific Coelenterazine Analogs for Bioassays and Molecular Imaging.
Bioconjug Chem
September 2024
Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu, Tokyo 182-8585, Japan.
Bioluminescence (BL) generated by luciferase-coelenterazine (CTZ) reactions is broadly employed as an optical readout in bioassays and in vivo molecular imaging. In this study, we demonstrate a systematic approach to elucidate the luciferase-CTZ binding chemistry with a full set of regioisomeric CTZ analogs, where all the functional groups were regiochemically modified. When the chemical structures were categorized into Groups 1-6, the even-numbered Groups (2, 4, and 6) of the CTZ analogs are found to be exceptionally bright with NanoLuc enzyme.
View Article and Find Full Text PDFAntimicrobial and anticancer properties of carbon monoxide releasing molecules of the -[Re(CO)(N-N)L] family.
Dalton Trans
July 2024
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República (EAN), 2780-157 Oeiras, Portugal.
Article Synopsis
- - The study focuses on the toxicity of certain -[Re(CO)(N-N)L] complexes, emphasizing how changes to the axial ligand L can significantly impact their effectiveness against microbial and tumoral cells, unlike previous research which mainly examined modifications to the bidentate diimine ligand (N-N).
- - The high toxicity of the -[Re(CO)(bpy)(Ctz)] complex is linked to the structural presence of the trityl group in clotrimazole, and modifications to the axial ligand maintain high activity levels, suggesting ligand structure is key in inhibiting bacterial cell processes.
- - Compounds containing PhE groups demonstrated tenfold increased toxicity compared to cisplatin in mammalian cells, with the
Surface modification of neurovascular stents: from bench to patient.
J Neurointerv Surg
August 2024
New England Center for Stroke Research, Department of Radiology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA
Flow-diverting stents (FDs) for the treatment of cerebrovascular aneurysms are revolutionary. However, these devices require systemic dual antiplatelet therapy (DAPT) to reduce thromboembolic complications. Given the risk of ischemic complications as well as morbidity and contraindications associated with DAPT, demonstrating safety and efficacy for FDs either without DAPT or reducing the duration of DAPT is a priority.
View Article and Find Full Text PDFBiophysical analysis of luciferase bioluminescence mechanisms using a non-oxidizable coelenterazine.
BBA Adv
December 2022
Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei-shi, Tokyo 184-8588, Japan.
luciferase (GLuc 18.2kDa; 168 residues) is a marine copepod luciferase that emits a bright blue light when oxidizing coelenterazine (CTZ). It is a helical protein where two homologous sequential repeats form two anti-parallel bundles, each made of four helices.
View Article and Find Full Text PDFBioluminescent imaging systems boosting near-infrared signals in mammalian cells.
Photochem Photobiol Sci
June 2023
Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu, Tokyo, 182-8585, Japan.
Bioluminescence (BL) is broadly used as an optical readout in bioassays and molecular imaging. In this study, the near-infrared (NIR) BL imaging systems were developed. The system was harnessed by prototype copepod luciferases, artificial luciferase 30 (ALuc30) and its miniaturized version picALuc, and were characterized with 17 kinds of coelenterazine (CTZ) analogues carrying bulky functional groups or cyanine 5 (Cy5).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!