Knowledge about the genetic pathways that control nephron development is essential for better understanding the basis of congenital malformations of the kidney. The transcription factors Osr1 and Hand2 are known to exert antagonistic influences to balance kidney specification. Here, we performed a forward genetic screen to identify nephrogenesis regulators, where whole genome sequencing identified an lesion in the novel () mutant. The characterization of the mutant revealed that is needed to specify not renal progenitors but rather their maintenance. Additionally, promotes the expression of in the intermediate mesoderm (IM) and later the podocyte lineage. deficiency reduced podocytes, where overexpression of was sufficient to rescue podocytes and deficiency. Antagonism between and mediates podocyte development specifically by controlling expression. These studies reveal new insights about the roles of Osr1 in promoting renal progenitor survival and lineage choice.
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| http://dx.doi.org/10.3390/biomedicines10112868 | DOI Listing |
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