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Insecticide Susceptibility Status of and Mosquitoes in Malaria and Dengue Endemic Areas, Thai-Myanmar Border. | LitMetric

AI Article Synopsis

Article Abstract

The occurrence and spread of insecticide resistance has had a negative effect on the efficacy of insecticide-based tools and is distributed worldwide, including the Greater Mekong Subregion (GMS). This study aims to determine the insecticide susceptibility of malaria and dengue vectors in malaria and dengue hotspots on the Thai-Myanmar border. Mosquito larvae and pupae were obtained from water sources from December 2019 to April 2020 in Tha Song Yang District, Tak province, western Thailand. WHO bioassay susceptibility tests were conducted with three classes of insecticides to evaluate the knockdown and mortality rates of and female adults. V1016G and F1534C mutations in the voltage-gated sodium channel of were identified using a multiplex PCR. A total of 5764 female mosquitoes were bioassayed in this study, including spp. (92.63%) and F1 (7.37%). After 24 h of observation, s.l. ( = 3885) and s.l. ( = 1138) in Suan Oi (SO) and Tala Oka (TO) were susceptible to pyrethroids, organophosphates and carbamates (except bendiocarb) with 98-100% mortality (MR). Resistance to bendiocarb was detected with a mortality rate of 88.80%, 88.77%, and 89.92% for s.l. ( = 125, 125) and s.l. ( = 66), respectively. The first generation of . adult females were suspected of resistance to deltamethrin ( = 225, MR = 96.89%) and confirmed resistance to permethrin ( = 200, MR = 20.00%). V1016G and F1534C mutations were detected in three genotypes, heterozygote and homozygote forms. The correlation between the alleles and deltamethrin resistance was significant. In conclusion, bendiocarb resistance was found in primary malaria vectors, s.l. and s.l. F1 population was pyrethroids-resistant, associated with alleles. Therefore, molecular analysis should be conducted to gain insights into the mechanism of insecticide resistance. Routine malaria vector control programmes, such as fogging implementation in hotspot villages to induce resistance available in peri-domestic sites, are questionable.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694411PMC
http://dx.doi.org/10.3390/insects13111035DOI Listing

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