We present here new amperometric electrodes for the selective and quantitative detection of acetylcholine. The detection was achieved based on the electrodeposition of a carbon electrode with poly(3,4-ethylenedioxythiophene) (PEDOT) followed by the drop-casting of an ionophore-doped poly(vinyl) chloride (PVC) membrane. This work paves the way for future applied research to study neurological disorders.
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http://dx.doi.org/10.1039/d2cc03946j | DOI Listing |
bioRxiv
December 2024
State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China.
The neurotransmitter acetylcholine (ACh) is essential in both the central and peripheral nervous systems. Recent studies highlight the significance of interactions between ACh and various neuromodulators in regulating complex behaviors. The ability to simultaneously image ACh and other neuromodulators can provide valuable information regarding the mechanisms underlying these behaviors.
View Article and Find Full Text PDFFront Behav Neurosci
December 2024
Department of Biological Sciences, Lehigh University, Bethlehem, PA, United States.
Introduction: Anxiety disorders are among the most common mental illnesses in the US. An estimated 31.1% of U.
View Article and Find Full Text PDFElife
January 2025
Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Cigarette smoking is a well-known risk factor inducing the development and progression of various diseases. Nicotine (NIC) is the major constituent of cigarette smoke. However, knowledge of the mechanism underlying the NIC-regulated stem cell functions is limited.
View Article and Find Full Text PDFLife Sci
January 2025
State Key Laboratory of Natural Medicines, School of life science and technology, China Pharmaceutical University, Nanjing 211000, PR China. Electronic address:
Background And Purpose: Sepsis is a condition capable of causing systemic inflammation and metabolic reprogramming. Previous studies have shown that sinomenine (SIN) can mitigate sepsis by reducing inflammation, while the effect on metabolic reprogramming is unclear. The aim of this study is to investigate the function of SIN in metabolic reprogramming in sepsis.
View Article and Find Full Text PDFJ Biol Chem
December 2024
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, Japan. Electronic address:
The mechanistic target of rapamycin complex 1 (mTORC1) functions as a master regulator of cell growth and proliferation. We previously demonstrated that intracellular calcium ion (Ca) concentration modulates the mTORC1 pathway via binding of the Ca sensor protein calmodulin (CaM) to tuberous sclerosis complex 2 (TSC2), a critical negative regulator of mTORC1. However, the precise molecular mechanism by which Ca/CaM modulates mTORC1 activity remains unclear.
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