Cannabidiol (CBD) exhibits anti-inflammatory, anxiolytic, antiseizure, and neuroprotective proprieties without addictive or psychotropic side effects, as opposed to Δ-tetrahydrocannabinol (THC). While recreational cannabis contains higher THC and lower CBD concentrations, medical cannabis contains THC and CBD in different ratios, along with minor phytocannabinoids, terpenes, flavonoids and other chemicals. A volumetric absorptive microsampling (VAMS) method combined with ultra-high-performance liquid chromatography coupled with mass spectrometry in tandem for quantification of CBD, THC and their respective metabolites: cannabidiol-7-oic acid (7-COOH-CBD); 7-hydroxy-cannabidiol (7-OH-CBD); 6-alpha-hydroxy-cannabidiol (6-α-OH-CBD); and 6-beta-hydroxycannabidiol (6-β-OH-CBD); 11- Hydroxy-Δ-tetrahydrocannabinol (11-OH-THC) and 11-Nor-9-carboxy-Δ-tetrahydrocannabinol (THCCOOH). After overnight enzymatic glucuronide hydrolysis at 37°C, samples underwent acidic along with basic liquid-liquid extraction with hexane: ethyl acetate (9:1, v/v). Chromatographic separation was carried out on a C18 column, with the mass spectrometer operated in multiple reaction monitoring mode and negative electrospray ionization. Seven patients with intractable epilepsy were dosed with various CBD-containing formulations and blood collected just before their daily morning administration. The method was validated following international guidelines in toxicology. Linear ranges were (ng/ml) 0.5-25 THC, 11-OH-THC, THCCOOH, 6-α-OH-CBD and 6-β-OH-CBD; 10-500 CBD and 7-OH-CBD; and 20-5000 7-COOH-CBD. 7-COOH-CBD was present in the highest concentrations, followed by 7-OH-CBD and CBD. This analytical method is useful for investigating CBD, THC and their major metabolites in epilepsy patients treated with CBD preparations employing a minimally invasive microsampling technique requiring only 30 µL blood.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637868 | PMC |
| http://dx.doi.org/10.3389/fphar.2022.1038754 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT.
Heliyon
December 2024
Empa - Swiss Federal Laboratories for Materials Science and Technology, 8600, Dübendorf, Switzerland.
Rationale And Objective: The visualization of soft tissues, like the meniscus, through X-ray micro-computed tomography (micro-CT), requires the use of contrast agents (CAs). While other studies have investigated CA diffusion in fibrocartilagineous tissues, this work aimed to optimize iodine staining protocols for meniscal tissue that improve their visualization by micro-CT. Specific objectives included evaluating the diffusion of CAs within meniscal samples over time, assessing volume changes due to staining, and identifying the iodine ions absorbed by the tissue.
View Article and Find Full Text PDFDesign and evaluation of two proposed hybrid FCC-BCC lattice structures for enhanced mechanical performance.
Heliyon
January 2025
Department of Mechanical Engineering, Sharif University of Technology, Tehran, Iran.
Lattice structures are an innovative solution to increase the strength-to-weight ratio of a structure. In this study, two polymeric hybrid lattice structures-"FRB" (a heterogenous structure which is indeed a BCC structure reinforced by FCC unit cells dispersed in a way to form a chessboard pattern in each layer) and the "Multifunctional" (a homogenous structure whose unit cells are a combination of FCC and BCC unit cells where their central nodes are connected)-are proposed, fabricated via liquid crystal display 3D printing technique, and their mechanical characteristics are evaluated under quasi-static loading, experimentally and numerically. The results indicate a 15.
View Article and Find Full Text PDFDataset on hydrogeochemical characteristics of spring and surface waters in the complex karst catchment area of Southern Dalmatia (Croatia) and Western Herzegovina (Bosnia and Herzegovina).
Data Brief
December 2024
University of Zagreb, Faculty of Mining, Geology and Petroleum Engineering, Pierottijeva 6, HR-10000 Zagreb, Croatia.
Large and complex karst catchments, like the one in Southern Dalmatia (Croatia) and Western Herzegovina (Bosnia and Herzegovina), are fragile environments requiring careful protection and sustainable water resources management. Understanding the processes that influence karst aquifer water chemistry is essential for the effective protection of water quality and quantity, ensuring sustainable resource availability and minimizing vulnerability to contamination. A hydrogeochemical dataset comprising over 30 groundwater (springs) and surface water samples, was collected in this cross-border catchment area from September 2013 to September 2020, accounting for seasonal variations.
View Article and Find Full Text PDFReplacing serum with dried blood microsampling for pharmacokinetics, viral neutralisation and immunogenicity bioanalysis supporting future paediatric development of RSM01, a candidate respiratory syncytial virus neutralising monoclonal antibody.
BMC Infect Dis
December 2024
Bill & Melinda Gates Medical Research Institute, Cambridge, MA, USA.
Background: Virus neutralising antibodies in serum are considered key correlates of protection for vaccines and monoclonal antibodies against respiratory syncytial virus (RSV). RSM01 is a novel, highly-potent, half-life-extended and fully-human monoclonal antibody candidate targeting the RSV prefusion F protein. Currently in Phase 1 development, RSM01 is primarily being developed to potentially provide an effective and affordable RSV prevention strategy in low- and middle-income countries.
View Article and Find Full Text PDFDevelopment and validation of a quantification method for direct oral anticoagulants from capillary blood using volumetric absorptive microsampling and online SPE-LC-MS.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Department of Pharmaceutical and Medical Chemistry, Clinical Pharmacy, University of Muenster, Muenster, Germany. Electronic address:
The number of prescriptions for new direct oral anticoagulants (DOACs) apixaban, edoxaban, rivaroxaban and dabigatran has increased exponentially in recent years, increasingly replacing the old gold standard, vitamin-K-antagonists. Due to their wide therapeutic range, therapeutic drug monitoring (TDM) is not required, although it has been proven that this could significantly reduce side effects. In order to develop a cost-efficient and simple method for the simultaneous detection of the DOACs and phenprocoumon, a new technology for sample preparation from capillary blood in the ambulant sector named VAMS® was integrated and an LC-MS detector with on-line solid phase extraction (SPE) applying a Turboflow HTLC Cyclone 1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!