Rapid and specific detection of and using a loop-mediated isothermal amplification assay.

and are the main causative pathogens of onychomycosis. However, the sensitivity and specificity of conventional microscopic tests are insufficient for reliable diagnoses of onychomycosis. In this study, we developed loop-mediated isothermal amplification (LAMP) assays for the rapid and specific identification of the two major spp. We designed LAMP primers targeting the internal transcribed spacer 1 region of the and . Through rigorous optimization of the reaction conditions, we defined a universal reaction condition for both LAMP assays.