High Prevalence of Infection in Fighting Cocks in Thailand Determined with a Molecular Method.

J Vet Res

One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham 44000, Thailand.

Published: September 2022

Introduction: Avian malaria caused by and the malaria-like parasites of the genus has been regularly described in multiple regions worldwide. These parasites significantly affect many avian taxa, including domestic chickens and fighting cocks. There are limited epidemiological studies of these blood parasites in vertebrate hosts, especially in Thailand.

Material And Methods: This study used microscopic examination of blood samples and PCR amplification exploiting primers for nucleotide sequences of or species based on the cytochrome gene to determine the occurrence of spp. in fighting cocks.

Results: Examination of 249 blood samples of fighting cocks revealed that 41.37% (103/249) were positive for malaria by microscopic examination and 88.76% (221/249) were positive by DNA amplification. Sequencing and DNA analysis of 61 PCR products revealed that infection by was the most common avian malaria in fighting cocks in Thailand followed by infections by ; however, infection was not discovered.

Conclusion: This study indicated that plasmodiasis is widespread in fighting cocks in Thailand although the prevalence was not clearly determined; therefore, prevention and control strategies for these protozoa should be improved, especially those for avoiding vector exposure and eliminating mosquito breeding sites.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597944PMC
http://dx.doi.org/10.2478/jvetres-2022-0049DOI Listing

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