AI Article Synopsis
Article Abstract
A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology for repair of the introduced double-stranded DNA (dsDNA) breaks, both of which are replaceable via restriction digest.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753633 | PMC |
| http://dx.doi.org/10.1128/mra.00833-22 | DOI Listing |
Publication Analysis
Top Keywords
clostridioides difficile
8
mutagenesis system
8
plasmid sequence
4
sequence availability
4
availability improved
4
improved clostridioides
4
difficile crispr-cas9
4
crispr-cas9 mutagenesis
4
system two-plasmid
4
two-plasmid mutagenesis
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!