Background: The bleaching procedure consists of chemical principles of free radical release that react with chromophores, which results in an amount of energy released in this process. However, the evaluation of the electrical potential generated in these protocols has not yet been thoroughly investigated in the literature. Thus, this study aimed to examine variations in pH, mV, and temperature of different concentrations of hydrogen peroxide in the presence or absence of an intermittent LED/LASER photo acceleration system.
Methods: The study was divided into six groups (n = 9) according to the concentration of hydrogen peroxide (6%, 15%, and 35%), associated or not with the photo acceleration system LED/LASER. We followed the variation of pH, mV, and temperature at 1, 5, 10, 15, and 30 min after gel manipulation. Data were evaluated by two-way ANOVA of repeated measures (α =0.05).
Results: pH, mV, and temperature of the groups showed statistical differences both in the light and bleach and in the interaction between the two factors (p < 0.0001), where pH and mV were more influenced by the bleach and light factor, while the temperature was influenced by the bleach factor associated with light. HP15 presented the most significant change in pH, mV, and temperature.
Conclusion: The use of LED/laser increased the temperature of the gels and altered the pH and mV kinetics of HP6 and HP15, which did not occur in HP35, possibly due to the high ionic potential linked to the concentration.
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http://dx.doi.org/10.1016/j.pdpdt.2022.103175 | DOI Listing |
Lab Chip
January 2025
Nanobioelectronics Laboratory (NBEL), Department of Biomedical Engineering, Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, 8410501 Beer Sheva, Israel.
Dissolved oxygen is crucial for metabolism, growth, and other complex physiological and pathological processes; however, standard physiological models (such as organ-on-chip systems) often use ambient oxygen levels, which do not reflect the lower levels that are typically found . Additionally, the local generation of reactive oxygen species (ROS; a key factor in physiological systems) is often overlooked in biology-mimicking models. Here, we present a microfluidic system that integrates electrochemical dissolved oxygen sensors with lab-on-a-chip technology to monitor the physiological oxygen concentrations and generate hydrogen peroxide (HO; a specific ROS).
View Article and Find Full Text PDFJ Chem Inf Model
January 2025
Department of Chemistry, University of Rome, Sapienza, P.le A. Moro 5, 00185 Rome, Italy.
The oxidation of Met residues in proteins is a complex process, where protein-specific structural and dynamical features play a relevant role in determining the reaction kinetics. Aiming to a full-side perspective, we report here a comprehensive characterization of Met oxidation kinetics by hydrogen peroxide in a leptin protein case study. To do that, we estimated the reaction-free energy profile of the Met oxidation via a QM/MM approach, while the kinetics of the formation of the reactive species were calculated using classical molecular dynamics (MD) simulations.
View Article and Find Full Text PDFBull Exp Biol Med
January 2025
Hunan University of Chinese Medicine, Changsha, Hunan, China.
We studied the effect of acteoside on a model of human corneal epithelial cells (HCEC) injury induced by HO. HCEC were divided into 4 groups and cultured for 24 h in normal medium (intact and control groups, respectively), or in a medium containing DMSO or 160 μM acteoside (DMSO and acteoside groups, respectively). Then, HO solution was added to HCEC for 4 h, except for intact cells.
View Article and Find Full Text PDFSci Rep
January 2025
Chemistry Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
In a quest to innovate biologically active molecules, the benzoylation of 4,6-dimethylpyrimidine-2-thiol hydrochloride (1) with benzoyl chloride derivatives was employed to produce a series of pyrimidine benzothioate derivatives (2-5). Subsequent sulfoxidation of these derivatives (2-5) using hydrogen peroxide and glacial acetic acid yielded a diverse array of pyrimidine sulfonyl methanone derivatives (6-9). In parallel, the sulfoxidation of pyrimidine sulfonothioates (10-12) yielded sulfonyl sulfonyl pyrimidines (13-15), originating from the condensation of compound 1 with sulfonyl chloride derivatives.
View Article and Find Full Text PDFActa Parasitol
January 2025
Department of Molecular Biology and Genetics, Ordu University, Ordu, Turkey.
Purpose: Acanthamoeba species are eucaryotic protozoa found predominantly in soil and water. They cause ulceration and vision loss in the cornea (Acanthamoeba keratitis) and central nervous system (CNS) infection involving the lungs (granulomatous amoebic encephalitis). Antiparasitic drugs currently used in the treatment of infections caused by Acanthamoeba species are not effective at the desired level in some anatomical regions such as the eye and CNS.
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