AI Article Synopsis
- The immunostained plaque assay uses specific antibodies to bind viral antigens, allowing for the detection of virus infectivity in cases where traditional staining methods fail.
- This technique is particularly useful for titrating viruses like wild-type West Nile virus (WNV-WT) and a replication-defective variant (WNV-ΔNS1).
- The method enhances the ability to quantify viral loads accurately, improving research on viruses that do not form typical plaques.
Article Abstract
Immunostained plaque assay based on the specific antibody binding to viral antigen enables the detection and titration of virus infectivity, especially for viruses that could not form plaques using the classical crystal violet or neutral red staining methods. Here we describe the application of this method to quantify viral titers of wild-type West Nile virus (WNV-WT) and replication-defective WNV-ΔNS1 virus.
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| http://dx.doi.org/10.1007/978-1-0716-2760-0_3 | DOI Listing |
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