AI Article Synopsis
- * Researchers conducted RNA sequencing and qRT-PCR to compare miR-99a-5p levels in HNSCC tissues and plasma EVs between patients with cancer and those with benign diseases.
- * Results indicated that plasma EVs miR-99a-5p could effectively differentiate HNSCC from benign conditions, with a notable diagnostic area under the curve (AUC) of 0.7494, suggesting it could be useful for early detection of HNSCC.
Article Abstract
This study aimed to investigate the applicability of plasma extracellular vesicles (EVs) miR-99a-5p as a potential head and neck squamous cell carcinoma (HNSCC) diagnostic biomarker. The miRNA expression of HNSCC tissue and plasma EVs were profiled by small RNA sequencing. qRT-PCR was performed to detect miR-99a-5p expression in HNSCC ( = 93) and benign disease ( = 39) plasma EVs and formalin-fixed and paraffin-embedded (FFPE) tissue ( = 110). We constructed receiver-operating characteristic curves to investigate the diagnostic efficiency of plasma EVs miR-99a-5p. Tumor tissue exhibited lower miR-99a-5p than para-tumor tissue. Patients with high miR-99a-5p expression exhibited significantly more p16 positive status. In contrast, HNSCC plasma EVs harbored more miR-99a-5p than the benign disease group. Plasma EVs miR-99a-5p distinguished HNSCC with area under the curve (AUC) of 0.7494 (95% CI: 0.6692-0.8296; < 0.0001), with 61.54% sensitivity and 75.27% specificity, respectively. Furthermore, plasma EVs miR-99a-5p also distinguished early HNSCC with AUC of 0.7394 (95% CI: 0.6284-0.8504; = 0.0002), with 79.07% sensitivity and 61.54% specificity, respectively. Plasma EVs miR-99a-5p is a potential biomarker for predicting early HNSCC.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9622758 | PMC |
| http://dx.doi.org/10.3389/pore.2022.1610699 | DOI Listing |
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