This study proposes a microplate assay for chemical toxicity evaluation using S. natans; this assay is a miniaturization of a culture dish assay from previous studies. The optimal initial frond number of S. natans in the microplate assay was established and the feasibility of the microplate assay was evaluated. To evaluate the applicability of the developed method, S. natans was exposed to six model chemicals (one metalloid and five heavy metals) and the specific growth rate and photosynthetic activity were compared. We determined that the optimal initial frond number was four fronds for the microplate assay. Additionally, toxicity sensitivity to six model chemicals revealed similar results to the culture dish assay. Therefore, we propose that the S. natans microplate assay is a simple and effective method to assess ecotoxicity as it requires less test solution and area than the culture dish assay. This methodology can be expandable to ecotoxicological research.
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| http://dx.doi.org/10.1016/j.marpolbul.2022.114274 | DOI Listing |
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