AI Article Synopsis
- Development of helical protein imprinted fibers (HPIFs) using zucchini-derived microcoils as carriers offers a novel approach for the selective recognition and purification of target proteins from complex biological samples.
- The method utilizes dopamine and 3,4-dihydroxyphenylacetic acid as biocompatible monomers inspired by mussel adhesive proteins, enhancing the effectiveness of the imprinted layer.
- Experiments demonstrate HPIFs' strong binding capabilities, indicating their practical use in protein purification with potential applications across food, chemical, and pharmaceutical industries.
Article Abstract
Highly selective recognition and purification of target proteins from complex biological matrices remains a challenging subject in natural and life sciences. Compared with natural recognition receptors, artificial imprinted polymers are an ideal alternative candidate. In this study, we report a novel method to prepare helical protein imprinted fibers (HPIFs) with zucchini-derived microcoils as a carrier, firstly. Inspired by the self-polymerization of adhesive proteins in mussels, dopamine and 3,4-dihydroxyphenylacetic acid were chosen as bifunctional monomers for the first time to form a biocompatible imprinted layer. The chemical/physical properties and recognition performance of HPIFs were studied in a series of experiments. Additionally, the practicability of HPIFs was verified by specifically recognizing target protein in complex egg white sample. The one-step synthesis process and excellent binding performance of HPIFs make them a promising material for protein recognition and purification, and endow HPIFs with potential application value in the food, chemical and pharmaceutical fields.
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| http://dx.doi.org/10.1016/j.foodchem.2022.134645 | DOI Listing |
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