AI Article Synopsis
- MicroRNAs (miRNAs) are short, noncoding RNAs (18-22 nucleotides) that play important roles in cellular communication and disease development.
- Extracellular vesicles (EVs) carry miRNAs and have better detection sensitivity and specificity than traditional serum biomarkers, making them valuable for liquid biopsies.
- This review discusses recent advances in EVs, including their sorting mechanisms, diagnostic potential, and technology for isolating EVs, while also highlighting challenges and future research directions for using EV-derived miRNAs in clinical applications.
Article Abstract
MicroRNAs (miRNAs) are a class of short, single-stranded, noncoding RNAs, with a length of about 18-22 nucleotides. Extracellular vesicles (EVs) are derived from cells and play a vital role in the development of diseases and can be used as biomarkers for liquid biopsy, as they are the carriers of miRNA. Existing studies have found that most of the functions of miRNA are mainly realized through intercellular transmission of EVs, which can protect and sort miRNAs. Meanwhile, detection sensitivity and specificity of EV-derived miRNA are higher than those of conventional serum biomarkers. In recent years, EVs have been expected to become a new marker for liquid biopsy. This review summarizes recent progress in several aspects of EVs, including sorting mechanisms, diagnostic value, and technology for isolation of EVs and detection of EV-derived miRNAs. In addition, the study reviews challenges and future research avenues in the field of EVs, providing a basis for the application of EV-derived miRNAs as a disease marker to be used in clinical diagnosis and even for the development of point-of-care testing (POCT) platforms.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618890 | PMC |
| http://dx.doi.org/10.3389/fbioe.2022.948959 | DOI Listing |
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