AI Article Synopsis
- - HiEFs (hinny embryonic fibroblasts) exhibit slower proliferation compared to other fibroblasts (HEFs, DEFs, MuEFs), possibly tied to gene expression differences.
- - Research using various methods indicated that HiEFs have significantly lower expression of the IGF2R gene, which is linked to cell proliferation.
- - The lower expression of IGF2R in HiEFs is confirmed to be a factor in their slower growth and is not due to abnormal imprinting of the gene.
Article Abstract
Background: Proliferation of embryonic fibroblasts under the same cell culture conditions, hinny embryonic fibroblasts (HiEFs) was slower than horse embryonic fibroblast (HEFs), donkey embryonic fibroblasts (DEFs) and mule embryonic fibroblasts (MuEFs). The imprinted genes IGF2 and IGF2R are important for cell proliferation. Therefore, we investigated whether the slower proliferation of HiEFs is related to an aberrant gene expression of IGF2 or its receptors or genes influencing the expression of the IGF2 system.
Methods And Results: Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P < 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs.
Conclusions: The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.
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| http://dx.doi.org/10.1007/s11033-022-07937-6 | DOI Listing |
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