A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational settings. Here we present a low-cost morbidostat called the EVolutionary biorEactor (EVE) that can be built by students with minimal engineering and programming experience. We describe how we validated EVE in a real classroom setting by evolving replicate populations under chloramphenicol challenge, thereby enabling students to learn about bacterial growth and antibiotic resistance.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9683782 | PMC |
| http://dx.doi.org/10.7554/eLife.83067 | DOI Listing |
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