Effects of Different Preparation Methods on Microbiota Composition of Fecal Suspension.

Mol Biotechnol

The Affiliated Lihuili Hospital, Ningbo University, Ningbo, 315046, Zhejiang, People's Republic of China.

Published: June 2023

AI Article Synopsis

  • Fecal microbiota transplantation (FMT) is a new therapy, but there's no standard method for preparing fecal suspensions, which are crucial for its success.
  • This study tested five different preparation methods on rat fecal samples to see how they affect the microbiota composition and diversity.
  • Results showed that all methods reduced bacterial richness compared to fresh feces, with varying levels of structural similarity to fresh microbiota, indicating that the choice of preparation method can influence the outcomes of FMT.

Article Abstract

Fecal microbiota transplantation is an emerging disease-modifying therapy. The viability of the microbiome in feces and its successful transfer depends on the preparation of fecal microbiota suspension. However, currently, no standard operation procedure is proposed for fecal suspension preparation. This study aims to compare the effect of different preparation methods on the composition of fecal microbiota composition in the rat. Four methods were used to collect the fecal suspension from fresh rat fecal (Group A), including stirring with normal saline (Group B), stirring with normal saline and then standing (Group C), stirring with normal saline and filtered with gauze (Group D), and stirring with normal saline and centrifuged (Group E). 16S ribosomal RNA gene (16S rDNA) sequencing technology was used to analyze the microbiota diversity and composition of each group of samples. Compared with fresh feces, the bacterial richness of the fecal suspension obtained by the four methods was significantly decreased (P < 0.05). The structural similarity with fresh fecal microbiota from high to low is groups B, D, C, and E. All four methods changed the microbiota structure to varying degrees, thus may affect the effect of FMT. In conclusion, choosing different methods to prepare fecal suspensions may help to better optimize the application of FMT.

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Source
http://dx.doi.org/10.1007/s12033-022-00590-1DOI Listing

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