Automated Layer Identification Method for Skin Tissue Histology Images.

We present a novel automated tissue layer identification method for histology images. The method requires a single user input: the number of layers to be identified. The method incorporates a coarse boundary identification step followed by a refinement step. The coarse identification segments the image into 125 × 125 pixel sub-tiles, computes the histogram of each sub-tile, implements K-means clustering to label each sub-tile, and uses Dijkstra's algorithm to form the layer boundary. The refinement step identifies hair follicles, improves the detail and accuracy of the boundary, and segments the epidermis. The method only uses one color channel (blue). We test our proposed method using eight excised porcine tissue samples taken at different anatomical locations. The layer segmentations demonstrated that the dermis thickness increased, and the subcutaneous thickness decreased moving from breast to belly. Minimal variation in the thickness of the epidermis layer across anatomical locations was observed. Overall, these results highlight the importance of quantifying and assessing the tissue environment. Moreover, we demonstrate that our proposed method was robust across different histology stains and did not depend on color-specific information.