Objective: is a secreted protein with protease activity, and its expression is associated with inflammatory airway diseases. This study aimed to investigate the role of and IL-13 in pediatric asthma.
Methods: In asthmatic and healthy children, the correlation between expression and blood IL-4, and IL-13 levels were investigated by serological analyses such as RT-qPCR and ELISA. The effects on the activity and apoptosis of bronchial epithelial cells following IL-13 stimulation were explored by the CCK-8 assay and flow cytometry, respectively. siRNA was used to knock down the expression level of bronchial epithelial cells and the effect of IL-13 stimulation on these cells was assessed by the CCK-8 assay and flow cytometry.
Results: , IL-4, and IL-13 were highly expressed in the serum of children with asthma. expression was highly correlated to serum IL-13. IL-13 stimulation reduced the activity of bronchial epithelial cells and promoted apoptosis. Lastly, knockdown of rescued the IL-13-induced decrease in activity and apoptosis.
Conclusion: is highly expressed in children with asthma and mediates the contributory effect of IL-13 on the occurrence and development of pediatric asthma.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597202 | PMC |
| http://dx.doi.org/10.3389/fped.2022.959439 | DOI Listing |
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