AI Article Synopsis
- The study focuses on the identification of protozoan species found in Madura cattle, which are important for enhancing meat self-sufficiency in Indonesia.
- Researchers collected and analyzed fecal samples from 100 Madura cattle using both morphological techniques and molecular methods, specifically PCR for DNA amplification.
- The findings revealed the presence of four different protozoan species, indicating a 21% occurrence rate, and suggested the need for further research on the diseases these protozoans may cause in the cattle to improve livestock management practices in the region.
Article Abstract
Background And Aim: In Indonesia, Madura cattle are native breeds that are expected to contribute to the improvement of regional meat self-sufficiency. spp. are protozoans that are commonly found in ruminants. This study aimed to identify the occurrence and diversity of spp. in Madura cattle.
Materials And Methods: In this study, fresh fecal samples were collected from 100 cattle in Kamal Subdistrict, Bangkalan District, Madura Island, Indonesia. Morphological detection was performed using a light microscope, and molecular identification was performed using a polymerase chain reaction. DNA amplification was conducted using various species-specific primers for , , , , , and .
Results: The results obtained 21% (21/100) of spp. based on morphological detection. A total of 15 positive samples with 500-25,000/mL oocysts were selected for DNA extraction and amplification, resulting in 12 positive samples. Four spp. were obtained based on molecular identification: , , , and .
Conclusion: Four species of namely , , , and were identified from fecal sample of Madura cattle using PCR method in this study. Further comprehensive studies are required to investigate the pathogenicity of spp. in Madura cattle. Therefore, improved and integrated management practices should be strengthened by local governments to prevent pathogenic diseases and increase national livestock productivity in Indonesia.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615507 | PMC |
| http://dx.doi.org/10.14202/vetworld.2022.2084-2088 | DOI Listing |
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