AI Article Synopsis
- Acute kidney injury (AKI) is a serious condition affecting the proximal tubules (PTs), but the mechanisms behind PT shedding and regeneration are not fully understood.
- Researchers analyzed single-cell RNA sequencing data to identify biomarkers for PT progenitor cells, employing various algorithms to assess their stemness and regeneration pathways.
- The study found that a specific PT cluster marked by Top2a significantly increases during AKI and plays a key role in kidney regeneration, with different subsets of progenitor cells activated based on the age and condition of the mice used in the experiments.
Article Abstract
Background: Acute kidney injury is a common fatal disease with complex etiology and limited treatment methods. Proximal tubules (PTs) are the most vulnerable segment. Not only in injured kidneys but also in normal kidneys, shedding of PTs often happens. However, the source cells and mechanism of their regeneration remain unclear.
Methods: ScRNA and snRNA sequencing data of acute injured or normal kidney were downloaded from GEO database to identify the candidate biomarker of progenitor of proximal tubules. SLICE algorithm and CytoTRACE analyses were employed to evaluate the stemness of progenitors. Then the repairing trajectory was constructed through pseudotime analyses. SCENIC algorithm was used to detect cell-type-specific regulon. With spatial transcriptome data, the location of progenitors was simulated. Neonatal/ adult/ aged mice and preconditioning AKI mice model and deconvolution of 2 RNA-seq data were employed for validation.
Results: Through cluster identification, PT cluster expressed Top2a specifically was identified to increase significantly during AKI. With relatively strong stemness, the Top2a-labeled PT cluster tended to be the origin of the repairing trajectory. Moreover, the cluster was regulated by Pbx3-based regulon and possessed great segmental heterogeneity. Changes of Top2a between neonatal and aged mice and among AKI models validated the progenitor role of Top2a-labeled cluster.
Conclusions: Our study provided transcriptomic evidence that resident proximal tubular progenitors labeled with Top2a participated in regeneration. Considering the segmental heterogeneity, we find that there is a group of reserve progenitor cells in each tubular segment. When AKI occurs, the reserve progenitors of each tubular segment proliferate and replenish first, and PT-progenitors, a cluster with no obvious PT markers replenish each subpopulation of the reserve cells.
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| http://dx.doi.org/10.1016/j.cellsig.2022.110506 | DOI Listing |
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