Genome mining for the search and discovery of two new globin-like enzymes, TriB fromFusarium poae and TutaA from Schizophyllum commne, are involved in the synthesis of two linear terpenes tricinonoic acid (1) and 2-butenedioic acid (3). Both in vivo heterologous biosynthesis and in vitro biochemical assays showed that these two enzymes catalyzed the C-C double bond cleavage of a cyclic sesquiterpene precursor (-)-germacrene D (7) and a linear diterpene backbone schizostain (2), respectively. Our work presents an unusual formation mechanism of linear terpenes from fungi and expands the functional skills of globin-like enzymes in the synthesis of terpene compounds.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S1875-5364(22)60192-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Secondary structure analysis of proteins within the same topology group.
Biochem Biophys Res Commun
November 2024
Department of Biophysics, Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russia.
The native conformation of a protein plays a decisive role in ensuring its functionality. It is established that the spatial structure of proteins may exhibit a greater degree of conservation than the corresponding amino acid sequences. This study aims to clarify structural distinctions between homologous and non-homologous proteins with identical topology.
View Article and Find Full Text PDFGenome mining of fungal globin-like enzymes for catalyzing the synthesis of linear terpenes.
Chin J Nat Med
October 2022
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. Electronic address:
Genome mining for the search and discovery of two new globin-like enzymes, TriB fromFusarium poae and TutaA from Schizophyllum commne, are involved in the synthesis of two linear terpenes tricinonoic acid (1) and 2-butenedioic acid (3). Both in vivo heterologous biosynthesis and in vitro biochemical assays showed that these two enzymes catalyzed the C-C double bond cleavage of a cyclic sesquiterpene precursor (-)-germacrene D (7) and a linear diterpene backbone schizostain (2), respectively. Our work presents an unusual formation mechanism of linear terpenes from fungi and expands the functional skills of globin-like enzymes in the synthesis of terpene compounds.
View Article and Find Full Text PDFImmunosuppressant mycophenolic acid biosynthesis employs a new globin-like enzyme for prenyl side chain cleavage.
Acta Pharm Sin B
November 2019
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Mycophenolic acid (MPA, ) and its derivatives are first-line immunosuppressants used in organ transplantation and for treating autoimmune diseases. Despite chemical synthetic achievements, the biosynthetic formation of a seven-carbon carboxylic acid pharmacophore side chain of , especially the processes involving the cleavage of the prenyl side chain between DHMP () and DMMPA (), remains unknown. In this work, we identified a membrane-bound prenyltransferase, PgMpaA, that transfers FPP to to yield FDHMP ().
View Article and Find Full Text PDFδ-Aminolevulinate induces fetal hemoglobin expression by enhancing cellular heme biosynthesis.
Exp Biol Med (Maywood)
October 2019
Department of Pediatrics, Augusta University, Augusta, GA 30912, USA.
Unlabelled: Sickle cell disease (SCD) and β-thalassemia are inherited blood disorders caused by genetic defects in the β-globin gene on chromosome 11, producing severe disease in people worldwide. Induction of fetal hemoglobin consisting of two α-globin and two γ-globin chains ameliorates the clinical symptoms of both disorders. In the present study, we investigated the ability of δ-aminolevulinate (ALA), the heme precursor, to activate γ-globin gene expression as well as its effects on cellular functions in erythroid cell systems.
View Article and Find Full Text PDFStructural characteristics that stabilize or destabilize different assembly levels of phycocyanin by urea.
Photosynth Res
July 2014
Schulich Faculty of Chemistry, Technion-Israel Institute of Technology Technion, Technion City, 32000, Haifa, Israel.
Phycocyanin is one of the two phycobiliproteins always found in the Phycobilisome antenna complex. It is always situated at the ends of the peripheral rods, adjacent to the core cylinders composed of allophycocyanin. The basic phycocyanin monomer is an (αβ) dimer of globin-like subunits with three covalently linked phycocyanobilin cofactors.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!