[Identification of terpene synthase gene family members in and its relationship with terpenoid metabolism].

Terpene synthase (TPS) plays important roles in the synthesis of terpenoids which are the main fragrances in flowers. To understand the function of genes in terpenoid metabolism in relation to flower aroma formation, we identified all gene family members in by analyzing its genome database. We then used a transcriptomic approach to analyze the differential gene expression patterns of gene family members in the scented flower compared to the non-scented flower 'Nova Zembla'. The contents of terpenoid compounds in petals of the above two species at different developmental stages were also measured by using qRT-PCR and head space-solid phase micro-extraction combined with gas chromatography-mass spectrometry. Our results showed that a total of 47 members, with individual lengths ranged from 591 to 2 634 bp, were identified in the genome. The number of exons in gene ranged from 3 to 12, while the length of each protein encoded ranged from 196 to 877 amino acids. Members of the family are mainly distributed in the chloroplast and cytoplasm. Phylogenetic analysis showed that genes can be clustered into 5 subgroups. Seven gene family members can be functionally annotated as gene family since they were temporally and spatially expressed as shown in the transcriptome data. Notably, , , and in were expressed highly in flower buds reached the peak in the full blossoming. Correlation analysis between gene expression levels and terpenoid content indicates that the expression levels of , , , and were positively correlated with the content of terpenoids in the petals of . at all flower developmental stages, suggesting that these six genes might be involved in the aroma formation in . .