Key Laboratory of Grassland Resources and Ecology of Western Arid Region, Ministry of Education, Key Laboratory of Grassland Resources and Ecology of Xinjiang, College of Grassland Science, Xinjiang Agricultural University, Urumqi, China.
Published: October 2022
AI Article Synopsis
Article Abstract
L. is an important legume forage grass with strong drought resistant, which could be utilized as an important gene pool in molecular breed of forage grass. In this study, seedlings were treated with 400 mM mannitol to simulate drought stress, and the morphological and physiological changes were investigated, as well as the transcriptome changes of seedlings at different treatment time points (0 h, 2 h, 6 h, 12 h, 24 h, 36 h and 48 h). Transcriptome analyses revealed four modules were closely related with drought response in by WGCNA analysis, and four transcription factor genes related with drought stress were identified (, , and ). Among them, was highly expressed in roots, and MfERF053 protein showed transcriptional activation activity by transient expression in tobacco leaves. Overexpression of in improved root growth, number of lateral roots and fresh weight under drought, salt stress and exogenous ABA treatments. Transgenic over-expressing gene grew significantly better than the wild type under both drought stress and salt stress when grown in soil. Taken together, our strategy with transcriptome combined WGCNA analyses identified key transcription factor genes from , and the selected gene was verified to be able to enhance drought and salt resistance when over-expressed in .
The origin of genes from non-coding sequences is a long-term and fundamental biological question. However, how de novo genes originate and integrate into the existing pathways to regulate phenotypic variations is largely unknown. Here, we selected seven genes from 782 de novo genes for functional exploration based on transcriptional and translational evidence.
Key Laboratory of Herbage Improvement and Grassland Agroecosystems, Lanzhou University, Lanzhou, 730020, China; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou, 730020, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China; Engineering Research Center of Grassland Industry, Ministry of Education, Gansu Tech Innovation Centre of Western China Grassland Industry, China. Electronic address:
Arbuscular mycorrhizal (AM) fungi are widely existing soil microorganisms that form symbiotic relationships with most terrestrial plants. They are important for enhancing adversity resistance, including resistance to disease and water stresses. Nevertheless, it is not clear whether the benefits can be maintained in regulating the occurrence of plant diseases under drought, flooding stress and during water restoration.
Maize ( L.) is a major food and feed crop and an important raw material for energy, chemicals, and livestock. The NF-Y family of transcription factors in maize plays a crucial role in the regulation of plant development and response to environmental stress.
Introduction: The identification of the physiological processes limiting carbon assimilation under water stress is crucial for improving model predictions and selecting drought-tolerant varieties. However, the influence of soil water availability on photosynthesis-limiting processes is still not fully understood. This study aimed to investigate the origins of photosynthesis limitations on potato () during a field drought experiment.
Drought is a persistent and serious threat to crop yield and quality. The identification and functional characterization of drought tolerance-related genes is thus vital for efforts to support the genetic improvement of drought-tolerant crops. Barley is highly adaptable and renowned for its robust stress resistance, making it an ideal subject for efforts to explore genes related to drought tolerance.
