Several virulence-related traits like pyocyanin are regulated by an intricate regulatory network called quorum sensing (QS) that relies on transcriptional regulators that are activated through binding to a self-produced molecule called an autoinducer (AI). QS is composed of three systems, Las, Rhl and Pqs. In the Las system, the regulatory protein LasR interacts with its AI to activate the other two QS systems. In turn, the Rhl and Pqs systems regulate the expression of multiple virulence-related genes, such as the genes of the reiterated operons and involved in pyocyanin production. The Las system also regulates the negative regulator RsaL, which provides negative feedback to the QS-response, including repression of pyocyanin synthesis genes. In this work, we describe that LasR can act as a negative regulator of transcription and hence of pyocyanin production and that this regulation is independent of RsaL activity. This work contributes to the understanding of QS-dependent pyocyanin production and demonstrates a previously uncharacterized role of LasR as a repressor.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/mic.0.001262 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Traditional Chinese Medicine Monomer Bakuchiol Attenuates the Pathogenicity of via Targeting PqsR.
Int J Mol Sci
December 2024
Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau, College of Life Sciences, Yan'an University, Yan'an 716000, China.
As the antibiotic resistance of pathogens becomes increasingly severe, it is becoming more feasible to use methods that suppress the virulence of pathogens rather than exerting selective pressure on their growth. , a dangerous opportunistic pathogen, infects hosts by producing multiple virulence factors, which are regulated by quorum-sensing (QS) systems, including the systems, systems, and systems. This study used the chromosome transcription fusion reporter model to screen the traditional Chinese medicine monomer library and found that bakuchiol can effectively inhibit the system and related virulence phenotypes of , including the production of virulence factors (pyocyanin, hydrogen cyanide, elastase, and lectin) and motility (swarming, swimming, and twitching motility) without affecting its growth.
View Article and Find Full Text PDFExploring the antivirulence potential of phenolic compounds to inhibit quorum sensing in Pseudomonas aeruginosa.
World J Microbiol Biotechnol
January 2025
Food Research Center (FoRC), Laboratory of Food Microbiology, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, SP, Brazil.
Bacteria coordinate gene expression in a cell density-dependent manner in a communication process called quorum sensing (QS). The expression of virulence factors, biofilm formation and enzyme production are QS-regulated phenotypes that can interfere in human health. Due to this importance, there is great interest in inhibiting QS, comprising an anti-virulence strategy.
View Article and Find Full Text PDFEvaluation of Antibacterial Activity in Some Algerian Essential Oils and Selection of Thymus vulgaris as a Potential Biofilm and Quorum Sensing Inhibitor Against Pseudomonas aeruginosa.
Chem Biodivers
January 2025
Laboratory of Molecular and Cellular Screening Processes at Centre of Biotechnology of Sfax, Sfax, Tunisia.
Biofilm formation and virulence factor production by Pseudomonas aeruginosa are identified as the main mechanisms of its antibiotic resistance and pathogenicity. In this context, the study of the chemical composition of three Algerian essential oils (EOs) and the screening of their antibacterial, antibiofilm, and virulence factor inhibitory activities enabled us to select the thyme EO as the best oil to control the P. aeruginosa strain isolated from hospital environments.
View Article and Find Full Text PDFComparative phenotypic and proteomic analysis of colistin-exposed .
Germs
September 2024
PhD, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam, and Research Center for Infectious Diseases, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam.
Introduction: The emergence of colistin resistance threatens the treatment of infections.
Methods: In this study, in vitro development of colistin resistance was investigated using comparative phenotypic and proteomic analysis of ATCC 9027, its 14-day colistin sub-MIC exposed strain (Col-E1), and 10-day antibiotic-free cultured Col-E1 strain (Col-E2). Antibiotic susceptibility, morphology, virulence factors, and proteomic changes were assessed using disc-diffusion, agar-based, spectrophotometry, SEM, and iTRAQ-LC-MS/MS methods.
Mayan Medicinal Plants and Demonstrate Anti-Infective Properties Against the Priority Antibiotic-Resistant Bacteria and .
Plants (Basel)
December 2024
Unidad Médica de Alta Especialidad, Centro Médico Ignacio García Téllez, Instituto Mexicano del Seguro Social, Mérida 97150, Yucatán, Mexico.
(1) Background: Carbapenem-resistant (CBRAB) and (CBRPA) are critical and high-priority pathogens that require new therapeutic developments. Medicinal plants are valuable pharmaceutical resources. This study explored the anti-infective properties of Mayan plants, , and .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!