Background: Bispecific antibodies (BsAb) belong to a novel antibody category with advantages over traditional mono-specific therapeutic antibodies. However, product variants are also commonly seen during the production of BsAb, which poses significant challenges to downstream processing. In this study, the adsorptive characteristics of a BsAb product and its variants were investigated for a set of depth filters during primary recovery of the cell culture fluid.
Methods: The retention of the BsAb product and its variants on a set of Millistak+® D0HC and X0HC depth filters were first investigated, followed by studying the mechanism of their adsorption on the depth filters. The chemical and structural properties of depth filters along with the molecular properties of the product and its variants were studied subsequently.
Results: The X0HC filter was found to be able to retain a significant amount of low molecular weight (LMW) variants along with a low amount of main product retained. Different levels of retention, observed for these variants, were correlated to their different hydrophobic and charge characteristics in relation with the adsorptive properties of the depth filters used. Electrostatic, hydrophobic, and hydrogen bonding interactions were found to be the key forces to keep product variants retained on the depth filter where the higher hydrophobicity of the LMW variants may cause them to be preferentially retained.
Conclusion: Harvest depth filters potentially can be utilized for retaining the BsAb variants, which depends on relative molecular properties of the product and its variants and adsorptive properties of the depth filters used.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590317 | PMC |
| http://dx.doi.org/10.1093/abt/tbac023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Estimating soil profile salinity under vegetation cover based on UAV multi-source remote sensing.
Sci Rep
January 2025
Research Center of Fluid Machinery Engineering and Technology, Jiangsu University, Zhenjiang, 212013, China.
Soil salinization is the most prevalent form of land degradation in arid, semi-arid, and coastal regions of China, posing significant challenges to local crop yield, economic development, and environmental sustainability. However, limited research exists on estimating soil salinity at different depths under vegetation cover. This study employed field-controlled soil experiments to collect multi-source remote sensing data on soil salt content (SSC) at varying depths beneath barley growth.
View Article and Find Full Text PDFRange and accuracy of in-plane anisotropic thermal conductivity measurement using the laser-based Ångstrom method.
Rev Sci Instrum
January 2025
Birck Nanotechnology Center and the School of Mechanical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
High heat fluxes in electronic devices must be effectively dissipated to prevent local hotspots, which are critical for long-term device reliability. In particular, advanced semiconductor packaging trends toward thin form factor products increase the need for understanding and improving in-plane conduction heat spreading in anisotropic materials. The 2D laser-based Ångstrom method, an extension of traditional Ångstrom and lock-in thermography techniques, measures in-plane thermal properties of anisotropic sheet-like materials.
View Article and Find Full Text PDFCharacterisation of the submarine disposal of a Bayer effluent (Gardanne alumina plant, southern France): III: Trace metals in the seawater column in the vicinity of the discharge area in the Mediterranean Sea.
Mar Pollut Bull
January 2025
CREOCEAN, Valparc- bât B, 230 avenue de Rome, 83500 La Seyne-sur-Mer, France.
In the context of evaluating the environmental impact of deep-sea tailing practices, we conducted a case study on the Bayer effluent released into the Mediterranean Sea by the French Gardanne alumina plant. This effluent results from the filtration of red mud, which has previously been discharged into the Cassidaigne canyon for 55 years. In 2015, regulatory changes permitted the released of a filtered effluent instead of the slurry.
View Article and Find Full Text PDFAUTOGRAPH: Chemical Reaction Networks in 3D.
J Chem Inf Model
January 2025
Theory and Simulation of Complex Systems, Institute of Physical Chemistry, Heinrich-Heine Universität, Universitätsstr. 1, 40225 Düsseldorf, Germany.
Understanding and analyzing large-scale reaction networks is a fundamental challenge due to their complexity and size, often beyond human comprehension. In this paper, we introduce AUTOGRAPH, the first web-based tool designed for the interactive three-dimensional (3D) visualization and construction of reaction networks. AUTOGRAPH emphasizes ease of use, allowing users to intuitively build, modify, and explore individual reaction networks in real time.
View Article and Find Full Text PDFDetection of functional diversity gradients and their geoclimatic filters is sensitive to data types (occurrence vs. abundance) and spatial scales (sites vs. regions).
Plant Divers
November 2024
Laboratório de Ciências Ambientais, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.
Functional diversity (FD) reflects within- and between-site variation of species traits (α- and β-FD, respectively). Understanding how much data types (occurrence-based vs. abundance-weighted) and spatial scales (sites vs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!