Antiglioma Natural Products from the Marine-Associated Fungus sp. ZZ1750.

Marine-derived fungi are one of the most important sources for the discovery of new bioactive natural products. This study characterized the isolation, structures, and antiglioma activities of twelve compounds, including three novel ones-penipyridinone B (), 11-(-)-penilloid A (), and 11,14-(+)-penilloid A ()-from the marine fungus sp. ZZ1750. The structures of the novel compounds were determined via extensive nuclear magnetic resonance (NMR) spectroscopic analyses, high-resolution electrospray ionization mass spectroscopy (HRESIMS) data, Mosher's method, optical rotation (OR) calculations, and electronic circular dichroism (ECD) calculations. Penipyridinone B represents the first example of its structural type and showed potent antiglioma activity, with IC values of 2.45 μM for U87MG cells and 11.40 μM for U251 cells. The known compounds of questiomycin A () and xanthocillin X () also showed antiproliferative activity against both U87MG and U251 cells, with IC values of 13.65 μM to 22.56 μM. The antiglioma activity of questiomycin A and xanthocillin X may be related to the promotion of reactive oxygen species (ROS) production, the reduction of mitochondrial membrane potential (MMP), and the enhancement of caspase-3 enzyme activity.