Evaluation of DNA-Damaging Effects Induced by Different Tanning Agents Used in the Processing of Natural Leather-Pilot Study on HepG2 Cell Line. | LitMetric
Division of Biology, Faculty of Science, University of Zagreb, Rooseveltov Trg 6, 10000 Zagreb, Croatia.
Published: October 2022
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For a long time, the production and processing of cowhide was based on the use of chrome tanning. However, the growing problem with chromium waste and its negative impact on human health and the environment prompted the search for more environmentally friendly processes such as vegetable tanning or aldehyde tanning. In the present study, we investigated the DNA-damaging effects induced in HepG2 cells after 24 h exposure to leather samples (cut into 1 × 1 cm rectangles) processed with different tanning agents. Our main objective was to determine which tanning procedure resulted in the highest DNA instability. The extent of treatment-induced DNA damage was determined using the alkaline comet assay. All tanning processes used in leather processing caused primary DNA damage in HepG2 cells compared to untreated cells. The effects measured in the exposed cells indicate that the leaching of potentially genotoxic chemicals from the same surface is variable and was highest after vegetable tanning, followed by synthetic tanning and chrome tanning. These results could be due to the complex composition of the vegetable and synthetic tanning agents. Despite all limitations, these preliminary results could be useful to gain a general insight into the genotoxic potential of the processes used in the processing of natural leather and to plan future experiments with more specific cell or tissue models.
Lung cancer is a prevalent and very aggressive sickness that will likely claim 1.8 million lives by 2022, with an estimated 2.2 million additional cases expected worldwide.
Bovine hair waste was chemically modified to obtain a coupling agent (CA) for the compatibilization of thermoplastic starch (TPS)-unmodified bovine hair waste (UH) composites. The composites processed with CA presented improved tensile strength (3.5 MPa) compared to TPS-UH composites without CA (1.
Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an 710129, China.
The utilization of chromium free tanning agents generates lots of pollutants such as chromium containing wastewater and sludge. Besides, trivalent chromium may be converted into carcinogenic hexavalent chromium under certain conditions, causing permanent harm to the human body. Therefore, the promotion of chromium free tanning technology is extremely significant.
To overcome the stringent regulations in the usage of chromium salts and dye-rich effluent let out by the tanning industry, a sustainable way of leather processing has been demonstrated utilizing amine pendant metal-organic frameworks (MOF) UiO-66-NH along with glyoxal. It was found that an offer of 8% (w/w) MOF along with 6% (w/w) glyoxal increased the shrinkage temperature of the leathers to 89 ± 2 °C with exhaustion of MOF up to 84.3 ± 1.
Facultad de Ciencias Exactas, Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Salta, 4400, Argentina.
Leather industry is traditionally characterized by the use of large amounts of chemical agents, some of which are toxic to human health and the environment. However, during the last years, many efforts have been made with the aim of successfully implement enzymes as agents for different leather production stages. The lipopeptides produced by the Bacillus spp.
