Oil palm empty fruit bunch (OPEFB) is the largest biomass waste from the palm oil industry. The OPEFB has a lignocellulose content of 34.77% cellulose, 22.55% hemicellulose, and 10.58% lignin. Therefore, this material's hemicellulose and cellulose content have a high potential for xylitol and ethanol production, respectively. This study investigated the integrated microaerobic xylitol production by and anaerobic ethanol semi simultaneous saccharification and fermentation (semi-SSF) by using the same OPEFB material. A maximum xylitol concentration of 2.86 g/L was obtained with a yield of 0.297 g/g. After 96 h of anaerobic fermentation, the maximum ethanol concentration was 6.48 g/L, corresponding to 71.38% of the theoretical ethanol yield. Significant morphological changes occurred in the OPEFB after hydrolysis and xylitol and ethanol fermentation were shown from SEM analysis.
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http://dx.doi.org/10.3390/microorganisms10102036 | DOI Listing |
Polymers (Basel)
December 2024
Department of Chemical, Environmental and Materials Engineering, Higher Polytechnical School of University of Jaén, Avda. de la Universidad s/n, 23700 Linares, Spain.
A sequential valorization process of sunflower stalks was carried out using nitric acid (0.1-2 mol dm) as a hydrolytic agent and fermenting the hydrolysate of higher sugar concentration in the presence of the non-conventional yeast . Values reached for ethanol yield (0.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2025
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
A novel yeast species, isolated from the bark of pine trees in Gyeongju, South Korea, and designated as KCTC 37304 (ex-type KACC 410729), is characterized by its genetic, morphological and physiological properties. Molecular phylogenetic analysis involving the D1/D2 domain of the 26S LSU rRNA gene and the internal transcribed spacer (ITS) region confirms that it belongs to the genus . In comparison to CBS:10065, the type strain of its closest relative, KCTC 37304 exhibits 8 nucleotide substitutions (~2.
View Article and Find Full Text PDFBioresour Technol
January 2025
Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; LABBELS -Associate Laboratory, Braga/Guimarães, Portugal. Electronic address:
The microbial biosynthesis of the antioxidant compound resveratrol offers an eco-friendly and sustainable alternative to chemical synthesis or plant extraction. Here, we showed that Kluyveromyces marxianus strains produce p-coumaric acid, a key precursor of resveratrol, with higher titres achieved under increased agitation conditions. Through further strain engineering, resveratrol production was achieved using glucose, xylose, and/or ethanol as substrates.
View Article and Find Full Text PDFFront Cell Infect Microbiol
August 2024
Institute of Medical Biology of the Polish Academy of Sciences, Łódź, Poland.
Introduction: The two-component signal transduction systems play an essential role in the adaptation of bacteria to changing environmental conditions. One of them is the MnoSR system involved in the regulation of methylotrophic metabolism in M. smegmatis.
View Article and Find Full Text PDFMetab Eng Commun
December 2024
Goethe University Frankfurt, Faculty of Biological Sciences, Institute of Molecular Biosciences, Max-von-Laue Straße 9, 60438, Frankfurt am Main, Germany.
Enhancing the supply of the redox cofactor NADPH in metabolically engineered cells is a critical target for optimizing the synthesis of many product classes, such as fatty acids or terpenoids. In , several successful approaches have been developed in different experimental contexts. However, their systematic comparison has not been reported.
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