AI Article Synopsis
- Sca-1CD31 cells are cardiac progenitor cells that can become heart muscle cells, and their behavior changes with age, particularly in relation to the gene Nr1d1.
- Experiments were conducted using techniques like lentiviral transduction to manipulate gene expression in Sca-1CD31 and mouse cardiac myocyte cells, examining effects on various cellular processes.
- Results showed that increasing the expression of the target gene inhibited cell growth and increased cell death in young cells, while reducing its expression in older cells had the opposite effect, indicating it may be a key player in aging and potential therapeutic target.
Article Abstract
Aim: Sca-1CD31 cells are resident cardiac progenitor cells, found in many mammalian tissues including the heart, and able to differentiate into cardiomyocytes in vitro and in vivo. Our previous work indicated that heart-derived Sca-1CD31 cells increased the Nr1d1 mRNA level of with aging. However, how affects the senescence of Sca-1CD31 cells.
Methods: Overexpression and knockdown of in Sca-1CD31 cells and mouse cardiac myocyte (MCM) cell lines were performed by lentiviral transduction. The effects of abundance on cell differentiation, proliferation, apoptosis, cell cycle, and transcriptomics were evaluated. Moreover, binding of to the promoter region of and was examined by a luciferase reporter assay.
Results And Conclusions: Upregulation in young Sca-1CD31 cells inhibited cell proliferation and promoted apoptosis. However, depletion of in aged Sca-1CD31 cells promoted cell proliferation and inhibited apoptosis. Furthermore, was negatively associated with cell proliferation, promoting apoptosis and senescence-associated beta-galactosidase production in MCMs. Our findings show that stimulates expression through its interaction with . may therefore act as a potent anti-aging receptor that can be a therapeutic target for aging-related diseases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603916 | PMC |
| http://dx.doi.org/10.3390/ijms232012455 | DOI Listing |
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