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Fast and Accurate Determination of Minute Ochratoxin A Levels in Cereal Flours and Wine with the Label-Free White Light Reflectance Spectroscopy Biosensing Platform. | LitMetric

AI Article Synopsis

  • Ochratoxin A (OTA) is a highly toxic contaminant found in various foods and beverages, prompting the development of quick and sensitive detection methods.
  • A new method using white light reflectance spectroscopy was created, which includes engineered silicon chips and antibodies to accurately measure OTA levels in cereal flours and wines.
  • This assay can detect OTA concentrations from 0.03 ng/mL to 200 ng/mL in just 25 minutes, demonstrating its effectiveness and ease of use in real-time monitoring for food safety.

Article Abstract

Ochratoxin A (OTA) is one of the most toxic naturally encountered contaminants and is found in a variety of foods and beverages, including cereals and wine. Driven by the strict regulations regarding the maximum allowable OTA concentration in foodstuff and the necessity for on-site determination, the development of fast and sensitive methods for the OTA determination in cereal flours and wine samples, based on white light reflectance spectroscopy, is presented. The method relied on appropriately engineered silicon chips, on top of which an OTA-protein conjugate was immobilized. A polyclonal antibody against OTA was then employed to detect the analyte in the framework of a competitive immunoassay; followed by the subsequent addition of a biotinylated secondary antibody and streptavidin for signal enhancement. A small size instrument performed all assay steps automatically and the bioreactions were monitored in real time as the software converted the spectral shifts into effective biomolecular adlayer thickness increase. The assay developed had a detection limit of 0.03 ng/mL and a working range up to 200 ng/mL. The assay lasted 25 min (less than 1h, including calibrators/antibody pre-incubation) and was accomplished following a simple sample preparation protocol. The method was applied to corn and wheat flour samples and white and red wines with recovery values ranging from 87.2 to 111%. The simplicity of the overall assay protocol and convenient instrumentation demonstrates the potential of the immunosensor developed for OTA detection at the point of need.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599867PMC
http://dx.doi.org/10.3390/bios12100877DOI Listing

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