AI Article Synopsis
- Arterial hypertension (HTN) poses a major public health issue, significantly contributing to cardiovascular diseases and kidney failure, with a specific focus on the overexpression of ENaC in human platelets as a key feature of HTN.
- In a double-blinded study with 167 participants, an innovative diagnostic assay using gold nanoparticles linked to an anti-ENaC antibody demonstrated a 62.1% success rate in identifying patients with known HTN and 59.7% in spotting previously undiagnosed cases.
- Despite some limitations in distinguishing between healthy individuals and HTN patients, this AuNP-anti-ENaC assay shows potential as a useful tool for early screening and improved management
Article Abstract
Arterial hypertension (HTN) is a global public health concern and an important risk factor for cardiovascular diseases and renal failure. We previously reported overexpression of ENaC on the plasma membrane of human platelets is a hallmark of HTN. In this double-blinded study of an open population ( = 167), we evaluated the sensitivity and specificity of a diagnostic assay based on gold nanoparticles (AuNPs) conjugated to an antibody against epithelial sodium channel (ENaC) expressed on platelets, which is detected using a fluorescent anti-ENaC secondary antibody and spectrofluorometry. Using the cutoff value for the AuNP-anti-ENaC assay, we confirmed the diagnosis for 62.1% of patients with clinical HTN and detected 59.7% of patients had previously undiagnosed HTN. Although some shortcomings in terms of accurately discriminating healthy individuals and patients with HTN still need to be resolved, we propose this AuNP-anti-ENaC assay could be used for initial screening and early diagnosis to critically improve opportune clinical management of HTN.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599886 | PMC |
| http://dx.doi.org/10.3390/bios12100806 | DOI Listing |
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